Reference Report

Reference
Citation	Powell, L.M., Deaton, A.M., Wear, M.A., Jarman, A.P. (2008). Specificity of Atonal and Scute bHLH factors: analysis of cognate E box binding sites and the influence of Senseless. Genes Cells 13(9): 915--929. (Export to RIS)
FlyBase ID	FBrf0215700
Publication Type	Research paper
PubMed ID	18681894
PubMed Abstract	The question of how proneural bHLH transcription factors recognize and regulate their target genes is still relatively poorly understood. We previously showed that Scute (Sc) and Atonal (Ato) target genes have different cognate E box motifs, suggesting that specific DNA interactions contribute to differences in their target gene specificity. Here we show that Sc and Ato proteins (in combination with Daughterless) can activate reporter gene expression via their cognate E boxes in a non-neuronal cell culture system, suggesting that the proteins have strong intrinsic abilities to recognize different E box motifs in the absence of specialized cofactors. Functional comparison of E boxes from several target genes and site-directed mutagenesis of E box motifs suggests that specificity and activity require further sequence elements flanking both sides of the previously identified E box motifs. Moreover, the proneural cofactor, Senseless, can augment the function of Sc and Ato on their cognate E boxes and therefore may contribute to proneural specificity.
DOI	10.1111/j.1365-2443.2008.01217.x
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genes Cells
Title	Genes to cells : devoted to molecular & cellular mechanisms
Publication Year	1996-
ISBN/ISSN	1356-9597
Data from Reference
Alleles (7)
	ato^Scer\UAS.cJa Avic\GFP^{atoE1x7.T:nls-tra} Avic\GFP^{hs.Stinger.T:nls-tra} Avic\GFP^{scE1x6.GGAA.T:nls-tra} Avic\GFP^{scE1x6.T:nls-tra} sc^Scer\UAS.cCa Scer\GAL4^sca-109-68
Constructs (5)
	P{atoE1x7-GFP} P{scE1x6-GFP.GGAA} P{scE1x6-GFP} P{UAS-ato.J} P{UAS-sc.C}
Genes (10)
	ato Avic\GFP Brd da E(spl)mγ-HLH sc Scer\GAL4 sens T:nls-tra Takr86C
Insertions (1)
	P{GawB}sca^109-68
Natural transposons (1)
	P-element