Reference Report

Reference
Citation	Matsubara, D., Horiuchi, S.Y., Shimono, K., Usui, T., Uemura, T. (2011). The seven-pass transmembrane cadherin Flamingo controls dendritic self-avoidance via its binding to a LIM domain protein, Espinas, in Drosophila sensory neurons. Genes Dev. 25(18): 1982--1996. (Export to RIS)
FlyBase ID	FBrf0215818
Publication Type	Research paper
PubMed ID	21937715
PubMed Abstract	Members of the Flamingo cadherin family are required in a number of different in vivo contexts of neural development. Even so, molecular identities downstream from the family have been poorly understood. Here we show that a LIM domain protein, Espinas (Esn), binds to an intracellular juxtamembrane domain of Flamingo (Fmi), and that this Fmi-Esn interplay elicits repulsion between dendritic branches of Drosophila sensory neurons. In wild-type larvae, branches of the same class IV dendritic arborization neuron achieve efficient coverage of its two-dimensional receptive field with minimum overlap with each other. However, this self-avoidance was disrupted in a fmi hypomorphic mutant, in an esn knockout homozygote, and in the fmi/esn trans-heterozygote. A functional fusion protein, Fmi:3eGFP, was localized at most of the branch tips, and in a heterologous system, assembly of Esn at cell contact sites required its LIM domain and Fmi. We further show that genes controlling epithelial planar cell polarity (PCP), such as Van Gogh (Vang) and RhoA, are also necessary for the self-avoidance, and that fmi genetically interacts with these loci. On the basis of these and other results, we propose that the Fmi-Esn complex, together with the PCP regulators and the Tricornered (Trc) signaling pathway, executes the repulsive interaction between isoneuronal dendritic branches.
DOI	10.1101/gad.16531611
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (31)
	Avic\GFP^1.E.ppk dsh¹ esn^f00447 esn^GD7841 esn^KO6 esn^{Scer\UAS.FL.T:Disc\RFP-mCherry} esn^{Scer\UAS.FL.T:Zzzz\FLAG} esn^{ΔLIM.Scer\UAS.T:Disc\RFP-mCherry} esn^{ΔLIM.Scer\UAS.T:Zzzz\FLAG} fry¹ fry⁰²²⁴⁰ fz²³ hpo^MGH4 Rho1^E3.10 Rho1^N19.Scer\UAS Scer\FLP1^SOP.scE1x6 Scer\GAL4⁴⁷⁷ Scer\GAL4^ptc-559.1 stan^{dsRNA.Scer\UAS.cMa} stan^E59 stan^E86 stan^Scer\UAS.AEY stan^{Scer\UAS.TTV.T:Avic\GFP-EGFP} stan^Scer\UAS.TTV stan^{ΔJM-A.Scer\UAS.TTV} T:Avic\GFP^EGFP T:Disc\RFP^mCherry trc¹ trc² Vang^A3 Vang^stbm-6
Constructs (13)
	P{GD7841} P{ppk-EGFP.1} P{SOP-FLP.scE1x6} P{UAS-esn.FL.mCherry} P{UAS-esn.FL} P{UAS-esn.ΔLIM.mCherry} P{UAS-esn.ΔLIM} P{UAS-fmi.AEY} P{UAS-fmi.RNAi} P{UAS-fmi.TTV.3eGFP} P{UAS-fmi.TTV} P{UAS-fmi.TTVΔJM-A} P{UAS-Rho1.N19}
Genes (15)
	Avic\GFP dsh esn fry futsch fz hpo pk Rho1 Scer\FLP1 Scer\GAL4 stan T:Zzzz\FLAG trc Vang
Insertions (1)
	P{GawB}ptc^559.1
Natural transposons (1)
	P-element