Reference Report

Reference
Citation	Davis, M.M., Primrose, D.A., Hodgetts, R.B. (2008). A member of the p38 mitogen-activated protein kinase family is responsible for transcriptional induction of Dopa decarboxylase in the epidermis of Drosophila melanogaster during the innate immune response. Mol. Cell. Biol. 28(15): 4883--4895. (Export to RIS)
FlyBase ID	FBrf0215839
Publication Type	Research paper
PubMed ID	18519585
PubMed Abstract	Drosophila innate immunity is controlled primarily by the activation of IMD (immune deficiency) or Toll signaling leading to the production of antimicrobial peptides (AMPs). IMD signaling also activates the JUN N-terminal kinase (JNK) cascade, which is responsible for immune induction of non-antimicrobial peptide immune gene transcription though the transcription factor AP-1. Transcription of the Dopa decarboxylase (Ddc) gene is induced in response to gram-negative and gram-positive septic injury, but not aseptic wounding. Transcription is induced throughout the epidermis and not specifically at the site of infection. Ddc transcripts are detectible within 2 h and remain high for several hours following infection with either gram-negative or gram-positive bacteria. Using Ddc-green fluorescent protein (GFP) reporter gene constructs, we show that a conserved consensus AP-1 binding site upstream of the Ddc transcription start site is required for induction. However, neither the Toll, IMD, nor JNK pathway is involved. Rather, Ddc transcription depends on a previously uncharacterized member of the p38 mitogen-activated protein kinase family, p38c. We propose that the involvement of DDC in a new pathway involved in Drosophila immunity increases the levels of dopamine, which is metabolized to produce reactive quinones that exert an antimicrobial effect on invading bacteria.
DOI	10.1128/MCB.02074-07
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Aberrations (1)
	Df(2L)TW130
Alleles (36)
	Avic\GFP^Ddc.BH Avic\GFP^Ddc.EH Avic\GFP^Ddc.PH Avic\GFP^{Ddc.PHmutAP-1} Avic\GFP^{Ddc.PHmutNFκB} Avic\GFP^Ddc.PHΔBE-1 Avic\GFP^Ddc.PHΔBE-2 Avic\GFP^Ddc.PHΔBE-3 Avic\GFP^Ddc.PHΔBE Avic\GFP^Ddc.SH Ddc⁷ Ddc^{dsRNA.Scer\UAS.Sym} Ddc^{dsRNA.Scer\UAS.WIZ} Ddc^ts2 dom^k08108 ird5^EY02434 ird5^KG08072 Mpk2¹ p38b^KG01337 p38c^1A1 p38c^2A p38c^4C p38c^7B1 p38c^8A p38c^19B1 p38c^KG05834 PGRP-LC^ΔE PGRP-LE¹¹² PGRP-SA^seml PGRP-SD^Δ3 Rel^EY08061 rl¹ Scer\GAL4^Act5C.PU Scer\GAL4^hs.PU Tak1^unspecified Tl³
Constructs (15)
	P{?SUPor-P} P{Act5C-GAL4.U} P{Ddc-GFP.BH} P{Ddc-GFP.EH} P{Ddc-GFP.PH} P{Ddc-GFP.PHmutAP-1} P{Ddc-GFP.PHmutNFκB} P{Ddc-GFP.PHΔBE-1} P{Ddc-GFP.PHΔBE-2} P{Ddc-GFP.PHΔBE-3} P{Ddc-GFP.PHΔBE} P{Ddc-GFP.SH} P{hs-GAL4.U} P{UAS-Ddc.RNAi.Sym} P{UAS-Ddc.RNAi.WIZ}
Genes (20)
	Avic\GFP bsk Ddc dom hep ird5 Jra kay Mpk2 p38b p38c PGRP-LC PGRP-LE PGRP-SA PGRP-SD Rel rl Scer\GAL4 Tak1 Tl
Insertions (4)
	P{?SUPor-P}p38c^1A1 P{?SUPor-P}p38c^7B1 P{?SUPor-P}p38c^19B1 P{SUPor-P}p38c^KG05834
Natural transposons (1)
	P-element