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| Citation | Miller, M.S., Lekkas, P., Braddock, J.M., Farman, G.P., Ballif, B.A., Irving, T.C., Maughan, D.W., Vigoreaux, J.O. (2008). Aging enhances indirect flight muscle fiber performance yet decreases flight ability in Drosophila. Biophys. J. 95(5): 2391--2401. (Export to RIS) | ||
| FlyBase ID | FBrf0215875 | ||
| Publication Type | Research paper | ||
| PubMed ID | 18515368 | ||
| PubMed Abstract | We investigated the effects of aging on Drosophila melanogaster indirect flight muscle from the whole organism to the actomyosin cross-bridge. Median-aged (49-day-old) flies were flight impaired, had normal myofilament number and packing, barely longer sarcomeres, and slight mitochondrial deterioration compared with young (3-day-old) flies. Old (56-day-old) flies were unable to beat their wings, had deteriorated ultrastructure with severe mitochondrial damage, and their skinned fibers failed to activate with calcium. Small-amplitude sinusoidal length perturbation analysis showed median-aged indirect flight muscle fibers developed greater than twice the isometric force and power output of young fibers, yet cross-bridge kinetics were similar. Large increases in elastic and viscous moduli amplitude under active, passive, and rigor conditions suggest that median-aged fibers become stiffer longitudinally. Small-angle x-ray diffraction indicates that myosin heads move increasingly toward the thin filament with age, accounting for the increased transverse stiffness via cross-bridge formation. We propose that the observed protein composition changes in the connecting filaments, which anchor the thick filaments to the Z-disk, produce compensatory increases in longitudinal stiffness, isometric tension, power and actomyosin interaction in aging indirect flight muscle. We also speculate that a lack of MgATP due to damaged mitochondria accounts for the decreased flight performance. | ||
| DOI | 10.1529/biophysj.108.130005 | ||
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| Language of Publication | English | ||
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| Publication Type | Journal | ||
| Abbreviation | Biophys. J. | ||
| Title | Biophysical Journal | ||
| Publication Year | 1960- | ||
| ISBN/ISSN | 0006-3495 | ||
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Data from Reference
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