Reference Report

Reference
Citation	Okajima, T., Reddy, B., Matsuda, T., Irvine, K.D. (2008). Contributions of chaperone and glycosyltransferase activities of O-fucosyltransferase 1 to Notch signaling. BMC Biol. 6(): 1. (Export to RIS)
FlyBase ID	FBrf0215952
Publication Type	Review
PubMed ID	18194540
PubMed Abstract	O-fucosyltransferase1 (OFUT1) is a conserved ER protein essential for Notch signaling. OFUT1 glycosylates EGF domains, which can then be further modified by the N-acetylglucosaminyltransferase Fringe. OFUT1 also possesses a chaperone activity that promotes the folding and secretion of Notch. Here, we investigate the respective contributions of these activities to Notch signaling in Drosophila.We show that expression of an isoform lacking fucosyltransferase activity, Ofut1R245A, rescues the requirement for Ofut1 in embryonic neurogenesis. Lack of requirement for O-fucosylation is further supported by the absence of embryonic phenotypes in Gmd mutants, which lack all forms of fucosylation. Requirements for O-fucose during imaginal development were evaluated by characterizing clones of cells expressing only Ofut1R245A. These clones phenocopy fringe mutant clones, indicating that the absence of O-fucose is functionally equivalent to the absence of elongated O-fucose.Our results establish that Notch does not need to be O-fucosylated for fringe-independent Notch signaling in Drosophila; the chaperone activity of OFUT1 is sufficient for the generation of functional Notch.
DOI	10.1186/1741-7007-6-1
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Language of Publication	English
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Publication Type	Journal
Abbreviation	BMC Biol.
Title	BMC Biology
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ISBN/ISSN	1741-7007
Data from Reference
Alleles (13)
	Avic\GFP^Scer\UAS.cUa Avic\GFP^{Scer\UAS.T:Uuuu\KDEL} fng^unspecified Gmd¹ m² O-fut1^4R6 O-fut1^{dsRNA.Scer\UAS} O-fut1^{R245A.Scer\UAS} O-fut1^R245A O-fut1^Scer\UAS.cOa Scer\GAL4^ap-md544 Scer\GAL4^ptc-559.1 Scer\GAL4^tub.PU
Constructs (7)
	P{O-fut1.R245A} P{tub-GAL4.U} P{UAS-GFP.KDEL} P{UAS-GFP.U} P{UAS-O-fut1.dsRNA} P{UAS-O-fut1.O} P{UAS-O-fut1.R245A}
Genes (10)
	Avic\GFP boca elav fng Gmd m N O-fut1 Scer\GAL4 wg
Insertions (2)
	P{GawB}ap^md544 P{GawB}ptc^559.1
Natural transposons (1)
	P-element