Open Close
Reference
Citation
Ramadan, N., Flockhart, I., Booker, M., Perrimon, N., Mathey-Prevot, B. (2007). Design and implementation of high-throughput RNAi screens in cultured Drosophila cells.  Nat. Protoc. 2(9): 2245--2264.
FlyBase ID
FBrf0216179
Publication Type
Research paper
Abstract

This protocol describes the various steps and considerations involved in planning and carrying out RNA interference (RNAi) genome-wide screens in cultured Drosophila cells. We focus largely on the procedures that have been modified as a result of our experience over the past 3 years and of our better understanding of the underlying technology. Specifically, our protocol offers a set of suggestions and considerations for screen optimization and a step-by-step description of the procedures successfully used at the Drosophila RNAi Screening Center for screen implementation, data collection and analysis to identify potential hits. In addition, this protocol briefly covers postscreen analysis approaches that are often needed to finalize the hit list. Depending on the scope of the screen and subsequent analysis and validation involved, the full protocol can take anywhere from 3 months to 2 years to complete.

PubMed ID
PubMed Central ID
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Protoc.
    Title
    Nature Protocols
    Publication Year
    2006--
    ISBN/ISSN
    1754-2189 1750-2799
    Data From Reference