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Citation
Venken, K.J., Schulze, K.L., Haelterman, N.A., Pan, H., He, Y., Evans-Holm, M., Carlson, J.W., Levis, R.W., Spradling, A.C., Hoskins, R.A., Bellen, H.J. (2011). MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes.  Nat. Methods 8(9): 737--743.
FlyBase ID
FBrf0216331
Publication Type
Research paper
Abstract
We demonstrate the versatility of a collection of insertions of the transposon Minos-mediated integration cassette (MiMIC), in Drosophila melanogaster. MiMIC contains a gene-trap cassette and the yellow+ marker flanked by two inverted bacteriophage ΦC31 integrase attP sites. MiMIC integrates almost at random in the genome to create sites for DNAmanipulation. The attP sites allow the replacement of the intervening sequence of the transposon with any other sequence through recombinase-mediated cassette exchange (RMCE). We can revert insertions that function as gene traps and cause mutant phenotypes to revert to wild type by RMCE and modify insertions to control GAL4 or QF overexpression systems or perform lineage analysis using the Flp recombinase system. Insertions in coding introns can be exchanged with protein-tag cassettes to create fusion proteins to follow protein expression and perform biochemical experiments. The applications of MiMIC vastly extend the D. melanogaster toolkit.
PubMed ID
PubMed Central ID
PMC3191940 (PMC) (EuropePMC)
Related Publication(s)
Supplementary material
Personal communication to FlyBase
set956.xml
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set958.xml
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set962.xml
Bellen, 2011.12.14, set962.xml [FBrf0216942]
set966.xml
Bellen, 2012.1.3, set966.xml [FBrf0217019]
set970.xml
Bellen, 2012.2.2, set970.xml [FBrf0217351]
set974.xml
Bellen, 2012.2.14, set974.xml [FBrf0217540]
set976.xml
Bellen, 2012.3.6, set976.xml [FBrf0217675]
set978.xml
Bellen, 2012.3.15, set978.xml [FBrf0217771]
set982.xml
Bellen, 2012.4.12, set982.xml [FBrf0218018]
set992.xml
Bellen, 2012.6.14, set992.xml [FBrf0218801]
set994.xml
Bellen, 2012.7.12, set994.xml [FBrf0218954]
set998.xml
Bellen, 2012.7.30, set998.xml [FBrf0219072]
set1002.xml
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set1005.xml
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set1006_v2.xml
Bellen, 2012.10.23, set1006_v2.xml [FBrf0219849]
set1011.xml
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set1016.xml
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set1020.xml
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set1026.xml
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Bellen, 2013.2.19, set1028.xml [FBrf0220967]
set1030_1032.xml
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set1036.xml
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set1042.xml
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set1046_1048.xml
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set1053.xml
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BDGP_2013_0925.xml
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BDGP_2013_1206.xml
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set1067.xml
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set1075.xml
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set1079.xml
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rel6freeze.xml
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set1099.xml
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set1114.xml
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set1116.xml
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set1121.xml
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set1124.xml
Bellen, 2015.2.4, set1124.xml [FBrf0227406]
set1126.xml
Bellen, 2015.2.9, set1126.xml [FBrf0227462]
set1128.xml
Bellen, 2015.2.19, set1128.xml [FBrf0227572]
set1112.xml
Bellen, 2015.4.17, set1112.xml [FBrf0228106]
set1131.xml
Bellen, 2015.4.24, set1131.xml [FBrf0228174]
set1125.xml
Bellen, 2015.6.6, set1125.xml [FBrf0228586]
setBCM050415.xml
Bellen, 2015.6.22, setBCM050415.xml [FBrf0228714]
set1118MI.xml
Bellen, 2015.7.29, set1118MI.xml [FBrf0229076]
MI03264.xml
Bellen, 2015.10.21, MI03264.xml [FBrf0229936]
MI05586.xml
Bellen, 2016.3.9, MI05586.xml [FBrf0231211]
BDGP_2010_0401.GDP.xml
Bellen, 2010.3.31, BDGP_2010_0401.GDP.xml [FBrf0210427]
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Bellen, 2010.4.14, BDGP_2010_0415.GDP.xml [FBrf0210544]
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Bellen, 2010.11.12, Set908.xml [FBrf0212197]
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set926.xml
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set930.xml
Bellen, 2011.3.29, set930.xml [FBrf0213349]
set932.xml
Bellen, 2011.5.24, set932.xml [FBrf0213718]
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Bellen, 2011.5.31, set938.xml [FBrf0213719]
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Note
'MiMICing' genomic flexibility.
Russell, 2011, Nat. Methods 8(9): 728--729 [FBrf0216337]
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Methods
    Title
    Nature Methods
    Publication Year
    2004-
    ISBN/ISSN
    1548-7091 1548-7105
    Data From Reference
    Alleles (60)
    Genes (19)
    Datasets (1)
    Molecular Constructs (24)
    Natural transposons (2)
    Insertions (52)
    Experimental Tools (19)
    Transgenic Constructs (27)