Reference Report

Reference
Citation	Venken, K.J., Schulze, K.L., Haelterman, N.A., Pan, H., He, Y., Evans-Holm, M., Carlson, J.W., Levis, R.W., Spradling, A.C., Hoskins, R.A., Bellen, H.J. (2011). MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. Nat. Methods 8(9): 737--743. (Export to RIS)
FlyBase ID	FBrf0216331
Publication Type	Research paper
PubMed ID	21985007
PubMed Abstract	We demonstrate the versatility of a collection of insertions of the transposon Minos-mediated integration cassette (MiMIC), in Drosophila melanogaster. MiMIC contains a gene-trap cassette and the yellow+ marker flanked by two inverted bacteriophage ΦC31 integrase attP sites. MiMIC integrates almost at random in the genome to create sites for DNAmanipulation. The attP sites allow the replacement of the intervening sequence of the transposon with any other sequence through recombinase-mediated cassette exchange (RMCE). We can revert insertions that function as gene traps and cause mutant phenotypes to revert to wild type by RMCE and modify insertions to control GAL4 or QF overexpression systems or perform lineage analysis using the Flp recombinase system. Insertions in coding introns can be exchanged with protein-tag cassettes to create fusion proteins to follow protein expression and perform biochemical experiments. The applications of MiMIC vastly extend the D. melanogaster toolkit.
DOI
Related Publication(s)
Supplementary material	Supplementary Material. [FBrf0218518]
Personal communication to FlyBase	set956.xml Bellen, 2011.10.25, set956.xml [FBrf0216550] set958.xml Bellen, 2011.11.10, set958.xml [FBrf0216694] set962.xml Bellen, 2011.12.14, set962.xml [FBrf0216942] set966.xml Bellen, 2012.1.3, set966.xml [FBrf0217019] set970.xml Bellen, 2012.2.2, set970.xml [FBrf0217351] set974.xml Bellen, 2012.2.14, set974.xml [FBrf0217540] set976.xml Bellen, 2012.3.6, set976.xml [FBrf0217675] set978.xml Bellen, 2012.3.15, set978.xml [FBrf0217771] set982.xml Bellen, 2012.4.12, set982.xml [FBrf0218018] set992.xml Bellen, 2012.6.14, set992.xml [FBrf0218801] set994.xml Bellen, 2012.7.12, set994.xml [FBrf0218954] set998.xml Bellen, 2012.7.30, set998.xml [FBrf0219072] set1002.xml Bellen, 2012.8.21, set1002.xml [FBrf0219255] set1005.xml Bellen, 2012.9.7, set1005.xml [FBrf0219418] set1006_v2.xml Bellen, 2012.10.23, set1006_v2.xml [FBrf0219849] set1011.xml Bellen, 2012.11.5, set1011.xml [FBrf0219886] set1016.xml Bellen, 2012.12.17, set1016.xml [FBrf0220305] set1020.xml Bellen, 2013.1.8, set1020.xml [FBrf0220461] set1024.xml Bellen, 2013.1.28, set1024.xml [FBrf0220602] set1026.xml Bellen, 2013.1.29, set1026.xml [FBrf0220603] set1028.xml Bellen, 2013.2.19, set1028.xml [FBrf0220967] set1030_1032.xml Bellen, 2013.3.22, set1030_1032.xml [FBrf0221062] BDGP_2010_0401.GDP.xml Bellen, 2010.3.31, BDGP_2010_0401.GDP.xml [FBrf0210427] BDGP_2010_0415.GDP.xml Bellen, 2010.4.14, BDGP_2010_0415.GDP.xml [FBrf0210544] Set908.xml Bellen, 2010.11.12, Set908.xml [FBrf0212197] BDGP_2010_1209.GDP.xml Bellen, 2010.12.6, BDGP_2010_1209.GDP.xml [FBrf0212438] set919.xml Bellen, 2011.1.31, set919.xml [FBrf0212923] set926.xml Bellen, 2011.2.28, set926.xml [FBrf0213112] set930.xml Bellen, 2011.3.29, set930.xml [FBrf0213349] set932.xml Bellen, 2011.5.24, set932.xml [FBrf0213718] set938.xml Bellen, 2011.5.31, set938.xml [FBrf0213719] Ambiguous sequence map data for four GDP lines. Levis, 2011.7.25, Ambiguous sequence map data for four GDP lines. [FBrf0214586] MI948.xml Bellen, 2011.9.26, MI948.xml [FBrf0216074] MISet953.xml Bellen, 2011.10.10, MISet953.xml [FBrf0216368]
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Nat. Methods
Title	Nature Methods
Publication Year	2004-
ISBN/ISSN	1548-7091 1548-7105
Data from Reference
Alleles (98)
	Arus\HRP^{CadN-MI00393-HS.0} Arus\HRP^{comm-MI00380-HS.1} Arus\HRP^{Rfx-MI00053-HS.1} Arus\HRP^{tutl-MI00290-HS.1} Arus\HRP^{wnd-MI00494-HS.2} Avic\GFP^{CadN-MI00393-BM.0} Avic\GFP^{CadN-MI00393-GFSTF.0} Avic\GFP^{comm-MI00380-BM.1} Avic\GFP^{comm-MI00380-GFSTF.1} Avic\GFP^{Rfx-MI00053-BM.1} Avic\GFP^{rhea-MI00296-BM.0} Avic\GFP^{rhea-MI00296-GFSTF.0} Avic\GFP^{tutl-MI00290-BM.1} Avic\GFP^{tutl-MI00290-GFSTF.1} Avic\GFP^{wnd-MI00494-BM.2} Avic\GFP^{wnd-MI00494-GFSTF.2} BM-40-SPARC^MI00329 BM-40-SPARC^MI00329-GAL4 CadN^MI00393 CadN^MI00393-BM.0 CadN^MI00393-DV.0 CadN^{MI00393-GFSTF.0} CadN^MI00393-HS.0 CadN^MI00393-KV.0 CadN^{MI00393-mCh.0} CadN^{MI00393-TRH.0} caps^MI00249 caps^MI00249-GAL4 comm^MI00380 comm^MI00380-BM.1 comm^MI00380-DV.1 comm^{MI00380-GFSTF.1} comm^MI00380-HS.1 comm^MI00380-KV.1 comm^{MI00380-mCh.1} comm^{MI00380-TRH.1} Disc\RFP^{CadN-MI00393-mCh.0} Disc\RFP^{comm-MI00380-mCh.1} Disc\RFP^{mCherry.10x.Scer\UAS} Disc\RFP^{mCherry.10xScer\UAS} Disc\RFP^{Rfx-MI00053-mCh.1} Disc\RFP^{rhea-MI00296-mCh.0} Disc\RFP^{tdTomato.Ncra\QUAS.T:Uuuu\MyrPalm2,T:Ivir\HA1} Disc\RFP^{tutl-MI00290-mCh.1} Disc\RFP^{wnd-MI00494-mCh.2} gogo^MI00065 gogo^MI00065-GAL4 MYPT-75D^MI00314 MYPT-75D^MI00314-GAL4 MYPT-75D^MI00314-QF Ncra\QF^{MYPT-75D-MI00314-QF} Rfx^MI00053 Rfx^MI00053-BM.1 Rfx^MI00053-DV.1 Rfx^MI00053-HS.1 Rfx^MI00053-KV.1 Rfx^{MI00053-mCh.1} Rfx^{MI00053-TRH.1} rhea^MI00296 rhea^MI00296-BM.0 rhea^MI00296-DV.0 rhea^{MI00296-GFSTF.0} rhea^MI00296-KV.0 rhea^{MI00296-mCh.0} rhea^{MI00296-TRH.0} Scer\GAL4^{BM-40-SPARC-MI00329-GAL4} Scer\GAL4^{caps-MI00249-GAL4} Scer\GAL4^{gogo-MI00065-GAL4} Scer\GAL4^{MYPT-75D-MI00314-GAL4} tutl^MI00290 tutl^MI00290-BM.1 tutl^MI00290-DV.1 tutl^{MI00290-GFSTF.1} tutl^MI00290-HS.1 tutl^MI00290-KV.1 tutl^{MI00290-mCh.1} wnd^MI00494 wnd^MI00494-BM.2 wnd^MI00494-DV.2 wnd^{MI00494-GFSTF.2} wnd^MI00494-HS.2 wnd^{MI00494-mCh.2} y^+mDint2 Zzzz\dendGFP^{CadN-MI00393-DV.0} Zzzz\dendGFP^{comm-MI00380-DV.1} Zzzz\dendGFP^{Rfx-MI00053-DV.1} Zzzz\dendGFP^{rhea-MI00296-DV.0} Zzzz\dendGFP^{tutl-MI00290-DV.1} Zzzz\dendGFP^{wnd-MI00494-DV.2} Zzzz\eqFP578^{CadN-MI00393-TRH.0} Zzzz\eqFP578^{comm-MI00380-TRH.1} Zzzz\eqFP578^{Rfx-MI00053-TRH.1} Zzzz\eqFP578^{rhea-MI00296-TRH.0} Zzzz\KR^{CadN-MI00393-KV.0} Zzzz\KR^{comm-MI00380-KV.1} Zzzz\KR^{Rfx-MI00053-KV.1} Zzzz\KR^{rhea-MI00296-KV.0} Zzzz\KR^{tutl-MI00290-KV.1}
Constructs (52)
	Mi{GT-FLPo} Mi{GT-GAL4} Mi{GT-QF} Mi{MIC} Mi{PT-BM.0} Mi{PT-BM.1} Mi{PT-BM.2} Mi{PT-DV.0} Mi{PT-DV.1} Mi{PT-DV.2} Mi{PT-GFSTF.0} Mi{PT-GFSTF.1} Mi{PT-GFSTF.2} Mi{PT-HS.0} Mi{PT-HS.1} Mi{PT-HS.2} Mi{PT-KV.0} Mi{PT-KV.1} Mi{PT-KV.2} Mi{PT-mCh.0} Mi{PT-mCh.1} Mi{PT-mCh.2} Mi{PT-TRH.0} Mi{PT-TRH.1} Mi{PT-TRH.2} P{QUAS-mtdTomato-3xHA} PBac{10xUAS-mCherry.V} PBac{10XUAS-mCherry} pBS-KS-attB1-2-GT-SA-Flpo-SV40 pBS-KS-attB1-2-GT-SA-GAL4-Hsp70pA pBS-KS-attB1-2-GT-SA-QF-Hsp70 pBS-KS-attB1-2-PT-SA-SD-0-Dendra-V5 pBS-KS-attB1-2-PT-SA-SD-0-EBFP2-3xMyc pBS-KS-attB1-2-PT-SA-SD-0-EGFP-FlAsH-StrepII-TEV-3xFlag pBS-KS-attB1-2-PT-SA-SD-0-HRP-S pBS-KS-attB1-2-PT-SA-SD-0-KillerRed-V5 pBS-KS-attB1-2-PT-SA-SD-0-mCherry pBS-KS-attB1-2-PT-SA-SD-0-TagRFP-T-3xHA pBS-KS-attB1-2-PT-SA-SD-1-Dendra-V5 pBS-KS-attB1-2-PT-SA-SD-1-EBFP2-3xMyc pBS-KS-attB1-2-PT-SA-SD-1-EGFP-FlAsH-StrepII-TEV-3xFlag pBS-KS-attB1-2-PT-SA-SD-1-HRP-S pBS-KS-attB1-2-PT-SA-SD-1-KillerRed-V5 pBS-KS-attB1-2-PT-SA-SD-1-mCherry pBS-KS-attB1-2-PT-SA-SD-1-TagRFP-T-3xHA pBS-KS-attB1-2-PT-SA-SD-2-Dendra-V5 pBS-KS-attB1-2-PT-SA-SD-2-EBFP2-3xMyc pBS-KS-attB1-2-PT-SA-SD-2-EGFP-FlAsH-StrepII-TEV-3xFlag pBS-KS-attB1-2-PT-SA-SD-2-HRP-S pBS-KS-attB1-2-PT-SA-SD-2-KillerRed-V5 pBS-KS-attB1-2-PT-SA-SD-2-mCherry pBS-KS-attB1-2-PT-SA-SD-2-TagRFP-T-3xHA
Genes (27)
	Arus\HRP Avic\GFP BM-40-SPARC CadN caps comm Disc\RFP gogo MYPT-75D Ncra\QF Rfx rhea Scer\GAL4 T:Hsap\MYC T:Ivir\HA1 T:SV5\V5 T:Uuuu\S T:Zzzz\FLAG T:Zzzz\StrepII T:Zzzz\TC T:Zzzz\TEV.CS tutl wnd y Zzzz\dendGFP Zzzz\eqFP578 Zzzz\KR
Insertions (52)
	Mi{GT-GAL4}BM-40-SPARC^MI00329-GAL4 Mi{GT-GAL4}caps^MI00249-GAL4 Mi{GT-GAL4}gogo^MI00065-GAL4 Mi{GT-GAL4}MYPT-75D^MI00314-GAL4 Mi{GT-QF}MYPT-75D^MI00314-QF Mi{MIC}BM-40-SPARC^MI00329 Mi{MIC}CadN^MI00393 Mi{MIC}caps^MI00249 Mi{MIC}comm^MI00380 Mi{MIC}gogo^MI00065 Mi{MIC}MYPT-75D^MI00314 Mi{MIC}Rfx^MI00053 Mi{MIC}rhea^MI00296 Mi{MIC}tutl^MI00290 Mi{MIC}wnd^MI00494 Mi{PT-BM.0}CadN^MI00393-BM.0 Mi{PT-BM.0}rhea^MI00296-BM.0 Mi{PT-BM.1}comm^MI00380-BM.1 Mi{PT-BM.1}Rfx^MI00053-BM.1 Mi{PT-BM.1}tutl^MI00290-BM.1 Mi{PT-BM.2}wnd^MI00494-BM.2 Mi{PT-DV.0}CadN^MI00393-DV.0 Mi{PT-DV.0}rhea^MI00296-DV.0 Mi{PT-DV.1}comm^MI00380-DV.1 Mi{PT-DV.1}Rfx^MI00053-DV.1 Mi{PT-DV.1}tutl^MI00290-DV.1 Mi{PT-DV.2}wnd^MI00494-DV.2 Mi{PT-GFSTF.0}CadN^{MI00393-GFSTF.0} Mi{PT-GFSTF.0}rhea^{MI00296-GFSTF.0} Mi{PT-GFSTF.1}comm^{MI00380-GFSTF.1} Mi{PT-GFSTF.1}tutl^{MI00290-GFSTF.1} Mi{PT-GFSTF.2}wnd^{MI00494-GFSTF.2} Mi{PT-HS.0}CadN^MI00393-HS.0 Mi{PT-HS.1}comm^MI00380-HS.1 Mi{PT-HS.1}Rfx^MI00053-HS.1 Mi{PT-HS.1}tutl^MI00290-HS.1 Mi{PT-HS.2}wnd^MI00494-HS.2 Mi{PT-KV.0}CadN^MI00393-KV.0 Mi{PT-KV.0}rhea^MI00296-KV.0 Mi{PT-KV.1}comm^MI00380-KV.1 Mi{PT-KV.1}Rfx^MI00053-KV.1 Mi{PT-KV.1}tutl^MI00290-KV.1 Mi{PT-mCh.0}CadN^{MI00393-mCh.0} Mi{PT-mCh.0}rhea^{MI00296-mCh.0} Mi{PT-mCh.1}comm^{MI00380-mCh.1} Mi{PT-mCh.1}Rfx^{MI00053-mCh.1} Mi{PT-mCh.1}tutl^{MI00290-mCh.1} Mi{PT-mCh.2}wnd^{MI00494-mCh.2} Mi{PT-TRH.0}CadN^{MI00393-TRH.0} Mi{PT-TRH.0}rhea^{MI00296-TRH.0} Mi{PT-TRH.1}comm^{MI00380-TRH.1} Mi{PT-TRH.1}Rfx^{MI00053-TRH.1}
Libraries/Collections (1)
	TI_set_Mi{MIC}.MI
Natural transposons (2)
	Dhyd\Minos Tni\piggyBac