Reference Report

Reference
Citation	Dubin-Bar, D., Bitan, A., Bakhrat, A., Amsalem, S., Abdu, U. (2011). Drosophila javelin-like encodes a novel microtubule-associated protein and is required for mRNA localization during oogenesis. Development 138(21): 4661--4671. (Export to RIS)
FlyBase ID	FBrf0216406
Publication Type	Research paper
PubMed ID	21989913
PubMed Abstract	Asymmetrical localization of mRNA transcripts during Drosophila oogenesis determines the anteroposterior and dorsoventral axes of the Drosophila embryo. Correct localization of these mRNAs requires both microtubule (MT) and actin networks. In this study, we have identified a novel gene, CG43162, that regulates mRNA localization during oogenesis and also affects bristle development. We also showed that the Drosophila gene javelin-like, which was identified based on its bristle phenotype, is an allele of the CG43162 gene. We demonstrated that female mutants for jvl produce ventralized eggs owing to the defects in the localization and translation of gurken mRNA during mid-oogenesis. Mutations in jvl also affect oskar and bicoid mRNA localization. Analysis of cytoskeleton organization in the mutants reveal defects in both MT and actin networks. We showed that Jvl protein colocalizes with MT network in Schneider cells, in mammalian cells and in the Drosophila oocyte. Both in the oocyte and in the bristle cells, the protein localizes to a region where MT minus-ends are enriched. Jvl physically interacts with SpnF and is required for its localization. We found that overexpression of Jvl in the germline affects MT-dependent processes: oocyte growth and oocyte nucleus anchoring. Thus, our results show that we have identified a novel MT-associated protein that affects mRNA localization in the oocyte by regulating MT organization.
DOI	10.1242/dev.069161
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (2)
	Df(3R)Exel6172 Df(3R)Exel6275
Alleles (15)
	Avic\GFP^{αTub67C.S65T.I167T.T:Btau\MAPT} Ecol\lacZ^KZ503 grk^2B6 grk^2E12 grk^HF jvl¹ jvl^f00590 jvl^GD11011 jvl^{RA.Scer\UAS.P\T.T:Avic\GFP} Khc::nod^143.2 Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} Scer\GAL4^neur-P72 Scer\GAL4^nos.PG Scer\GAL4^{nos.UTR.T:Hsim\VP16} spn-F^GD7489
Constructs (7)
	P{GAL4::VP16-nos.UTR} P{GAL4-nos.NGT} P{GD7489} P{GD11011} P{matα4-GAL-VP16} P{Tub67C-Tau-GFP} P{UASp-jvl.RA.GFP}
Genes (12)
	Avic\GFP bcd Ecol\lacZ grk ik2 jvl Khc::nod orb osk Scer\GAL4 spn-F T:Avic\GFP
Insertions (8)
	P{GAL4::VP16-nos.UTR}CG6325^MVD1 P{GAL4}neur^P72 P{GAL4-nos.NGT}40 P{KZ.TRAP}KZ503 P{matα4-GAL-VP16}V2H P{matα4-GAL-VP16}V37 P{NZ}143.2 P{Tub67C-Tau-GFP}2.1
Natural transposons (1)
	P-element