Reference Report

Reference
Citation	Shapira, S., Bakhrat, A., Bitan, A., Abdu, U. (2011). The Drosophila javelin Gene Encodes a Novel Actin-Associated Protein Required for Actin Assembly in the Bristle. Mol. Cell. Biol. 31(22): 4582--4592. (Export to RIS)
FlyBase ID	FBrf0216490
Publication Type	Research paper
PubMed ID	21930794
PubMed Abstract	The Drosophila melanogaster bristle is a highly polarized cell that builds specialized cytoskeletal structures. Whereas actin is required for increasing bristle length, microtubules are essential for bristle axial growth. To identify new proteins involved in cytoskeleton organization during bristle development, we focused on identifying and characterizing the javelin (jv) locus. We found that in a jv mutant, the bristle tip is swollen and abnormal organization of bristle grooves is seen over the entire bristle. Using confocal and electron microscopy, we found that in jv mutant bristles, actin bundles do not form properly due to a loss of actin filaments within the bundle. We show that jv is an allele of the predicted CG32397 gene that encodes a protein with no homologs outside insects. Expression of the Jv protein fused to a green fluorescent protein (GFP) shows that the protein is colocalized with actin bundles in the bristle. Moreover, expression of Jv-GFP within the germ line led to the formation of ectopic actin bundles that surround the nucleus of nurse cells. Thus, we report that Jv is a novel actin-associated protein required for actin assembly during Drosophila bristle development.
DOI	10.1128/MCB.05730-11
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Aberrations (10)
	Df(3L)BSC27 Df(3L)BSC224 Df(3L)BSC410 Df(3L)BSC411 Df(3L)BSC437 Df(3L)ED211 Df(3L)Exel6109 Df(3L)Exel7210 Df(3L)Exel8101 Df(3L)Exel9058
Alleles (17)
	CG8368^KK101144 CG8398^GD13915 CG18769^c03230 CG18769^EY01803 CG18769^GD446 jv¹ jv^c03230 jv^KK114510 jv^{Scer\UAS.P\T.cSa} jv^{Scer\UAS.P\T.T:Avic\GFP} ndl¹¹ ndl¹⁴ Scer\GAL4^Act5C.PU Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} Scer\GAL4^neur-P72 zpg⁹⁶ zpg¹⁰⁷
Constructs (8)
	P{Act5C-GAL4.U} P{GD446} P{GD13915} P{KK101144} P{KK114510} P{matα4-GAL-VP16} P{UASp-jv.GFP} P{UASp-jv.S}
Genes (12)
	CG8368 CG8398 CG18769 f hk jv ndl Scer\GAL4 sn spn-F T:Avic\GFP zpg
Insertions (8)
	P{GAL4}neur^P72 P{GD446}v9501 P{GD13915}v35927 P{GD13915}v35928 P{KK101144}VIE-260B P{KK114510}VIE-260B P{matα4-GAL-VP16}V37 PBac{PB}CG18769^c03230
Natural transposons (1)
	P-element