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Citation
Guruharsha, K.G., Rual, J.F., Zhai, B., Mintseris, J., Vaidya, P., Vaidya, N., Beekman, C., Wong, C., Rhee, D.Y., Cenaj, O., McKillip, E., Shah, S., Stapleton, M., Wan, K.H., Yu, C., Parsa, B., Carlson, J.W., Chen, X., Kapadia, B., Vijayraghavan, K., Gygi, S.P., Celniker, S.E., Obar, R.A., Artavanis-Tsakonas, S. (2011). A Protein Complex Network of Drosophila melanogaster.  Cell 147(3): 690--703.
FlyBase ID
FBrf0216491
Publication Type
Research paper
Abstract

Determining the composition of protein complexes is an essential step toward understanding the cell as an integrated system. Using coaffinity purification coupled to mass spectrometry analysis, we examined protein associations involving nearly 5,000 individual, FLAG-HA epitope-tagged Drosophila proteins. Stringent analysis of these data, based on a statistical framework designed to define individual protein-protein interactions, led to the generation of a Drosophila protein interaction map (DPiM) encompassing 556 protein complexes. The high quality of the DPiM and its usefulness as a paradigm for metazoan proteomes are apparent from the recovery of many known complexes, significant enrichment for shared functional attributes, and validation in human cells. The DPiM defines potential novel members for several important protein complexes and assigns functional links to 586 protein-coding genes lacking previous experimental annotation. The DPiM represents, to our knowledge, the largest metazoan protein complex map and provides a valuable resource for analysis of protein complex evolution.

Graphical Abstract
Obtained with permission from Cell Press.
PubMed ID
PubMed Central ID
PMC3319048 (PMC) (EuropePMC)
Related Publication(s)
Supplementary material
Personal communication to FlyBase

Refined DPiM dataset (DPiM-2) supersedes original unfiltered protein identification dataset (DPiM-1).
Guruharsha et al., 2013.7.9, Refined DPiM dataset (DPiM-2) supersedes original unfiltered protein identification dataset (DPiM-1). [FBrf0222073]

DPiM: the Drosophila Protein Interaction Mapping project
Mintseris et al., 2009.11.23, DPiM: the Drosophila Protein Interaction Mapping project [FBrf0209452]

FlyBase analysis

Rollback of original unfiltered protein interaction dataset (DPiM-1).
FlyBase curators, 2014, Rollback of original unfiltered protein interaction dataset (DPiM-1). [FBrf0226221]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
    Data From Reference