Reference Report

Reference
Citation	Stephan, R., Gohl, C., Fleige, A., Klämbt, C., Bogdan, S. (2011). Membrane-targeted WAVE mediates photoreceptor axon targeting in the absence of the WAVE complex in Drosophila. Mol. Biol. Cell 22(21): 4079--4092. (Export to RIS)
FlyBase ID	FBrf0216499
Publication Type	Research paper
PubMed ID	21900504
PubMed Abstract	A tight spatial-temporal coordination of F-actin dynamics is crucial for a large variety of cellular processes that shape cells. The Abelson interactor (Abi) has a conserved role in Arp2/3-dependent actin polymerization, regulating Wiskott-Aldrich syndrome protein (WASP) and WASP family verprolin-homologous protein (WAVE). In this paper, we report that Abi exerts nonautonomous control of photoreceptor axon targeting in the Drosophila visual system through WAVE. In abi mutants, WAVE is unstable but restored by reexpression of Abi, confirming that Abi controls the integrity of the WAVE complex in vivo. Remarkably, expression of a membrane-tethered WAVE protein rescues the axonal projection defects of abi mutants in the absence of the other subunits of the WAVE complex, whereas cytoplasmic WAVE only slightly affects the abi mutant phenotype. Thus complex formation not only stabilizes WAVE, but also provides further membrane-recruiting signals, resulting in an activation of WAVE.
DOI	10.1091/mbc.E11-02-0121
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Biol. Cell
Title	Molecular Biology of the Cell
Publication Year	1992-
ISBN/ISSN	1059-1524
Data from Reference
Aberrations (1)
	Df(3R)Exel8159
Alleles (40)
	Abi^EY20423 Abi^Scer\UAS.cSa Abi^{WAB-HHR.Scer\UAS} Abi^Δ20 Abi^ΔC.Scer\UAS Abi^ΔN.Scer\UAS Arpc1^unspecified Avic\GFP^{GMR.T:Myr-Src64B} Ecol\lacZ^{ro.T:Btau\MAPT} Ecol\lacZ^wg-17en40cP1 Hem^unspecified Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} SCAR^NIG.4636R SCAR^{Scer\UAS.P\T.cZa} SCAR^{Scer\UAS.T:Myr-Src64B} SCAR^unspecified SCAR^Δ37 SCAR^ΔB.Scer\UAS SCAR^{ΔB.ΔP.Scer\UAS} SCAR^ΔN.Scer\UAS SCAR^{ΔVCA.Scer\UAS.T:Myr-Src64B} SCAR^{ΔVCA.Scer\UAS} Scer\FLP1^ey.3.5.hs Scer\FLP1^ey.PN Scer\FLP1^hs.PG Scer\GAL4^c855 Scer\GAL4^da.G32 Scer\GAL4^elav-C155 Scer\GAL4^gcm.PU Scer\GAL4^GMR.long Scer\GAL4^GMR.PU Scer\GAL4^lama-A8 Scer\GAL4^repo Scer\GAL4^sca.PU Scer\GAL4^αTub84B.PL Scer\GAL80^αTub84B.PL twf^EP3701 WASp¹ WASp³ WASp^unspecified
Constructs (25)
	P{ey-FLP.3.5} P{ey-FLP.N} P{GAL4-da.G32} P{gcm-GAL4.U} P{GMR-GAL4.U} P{GMR-myr.GFP} P{hsFLP} P{longGMR-GAL4} P{NIG.4636R} P{ro.lacZ-tau.G} P{sca-GAL4.U} P{tubP-GAL4} P{tubP-GAL80} P{UAS-Ani.S} P{UAS-Ani.WAB-HHR} P{UAS-Ani.ΔC} P{UAS-Ani.ΔN} P{UAS-mCD8::GFP.L} P{UASp-SCAR.Z} P{UAS-SCAR.Myr} P{UAS-SCAR.ΔB.ΔP} P{UAS-SCAR.ΔB} P{UAS-SCAR.ΔN} P{UAS-SCAR.ΔVCA.Myr} P{UAS-SCAR.ΔVCA}
Genes (22)
	Abi Arpc1 Avic\GFP chp Ecol\lacZ elav ey Hem Mmus\Cd8a pros ro SCAR Scer\FLP1 Scer\GAL4 Scer\GAL80 sens Sra-1 svp T:Myr-Src64B twf WASp wg
Insertions (9)
	P{en1}wg^17en40cP1 P{EP}twf^EP3701 P{EPgy2}Abi^EY20423 P{GAL4}repo P{GawB}A8C P{GawB}elav^C155 P{GawB}l(2)c855^c855 P{UAS-SCAR.Myr}ZH-68E P{UAS-SCAR.ΔVCA.Myr}ZH-68E
Natural transposons (1)
	P-element