Reference Report

Reference
Citation	Port, F., Hausmann, G., Basler, K. (2011). A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion. EMBO Rep. 12(11): 1144--1152. (Export to RIS)
FlyBase ID	FBrf0216532
Publication Type	Research paper
PubMed ID	21886182
PubMed Abstract	Wnt proteins are secreted, lipid-modified glycoproteins that control animal development and adult tissue homeostasis. Secretion of Wnt proteins is at least partly regulated by a dedicated machinery. Here, we report a genome-wide RNA interference screen for genes involved in the secretion of Wingless (Wg), a Drosophila Wnt. We identify three new genes required for Wg secretion. Of these, Emp24 and Eclair are required for proper export of Wg from the endoplasmic reticulum (ER). We propose that Emp24 and Eca act as specific cargo receptors for Wg to concentrate it in forming vesicles at sites of ER export.
DOI	10.1038/embor.2011.165
Related Publication(s)
Note	Wnts need a p(assport)24 to leave the ER. Palmer et al., 2011, EMBO Rep. 12(12): 1207--1208 [FBrf0217809]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	EMBO Rep.
Title	EMBO Reports
Publication Year	2000-
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Data from Reference
Alleles (16)
	Avic\GFP^{EYFP-ER.Scer\UAS} CHOp24^GD3225 CHOp24^KK107384 cic^KK112641 eca^3R-45-33 eca^GD17660 eca^KK109376 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^Act5C.PI Scer\GAL4^ey.PU Scer\GAL4^GMR.PU Scer\GAL4^hh.PU Scer\GAL4^nub-AC-62 Scer\GAL4^{Scer\FRT.Act5C} Snx3^KK109353 wg^hs.2sev
Constructs (14)
	P{Act5C-GAL4} P{AyGAL4} P{ey-GAL4.U} P{GD3225} P{GD17660} P{GMR-GAL4.U} P{hh-GAL4.U} P{KK107384} P{KK109353} P{KK109376} P{KK112641} P{sev-wg.B} P{UAS-EYFP-KDEL} P{UAS-mCD8::GFP.L}
Genes (18)
	Avic\GFP bai CG9308 CG31787 CHOp24 cic dlp eca loj Mmus\Cd8a N opm p24-1 p24-2 Scer\GAL4 Snx3 wg wls
Insertions (1)
	P{GawB}nubbin-AC-62
Natural transposons (1)
	P-element