Reference Report

Reference
Citation	Eissenberg, J.C., Ilvarsonn, A.M., Sly, W.S., Waheed, A., Krzyzanek, V., Pohlmann, R., Waschkau, D., Kretzschmar, D., Dennes, A.C. (2011). Drosophila GGA Model: An Ultimate Gateway to GGA Analysis. Traffic 12(12): 1821--1838. (Export to RIS)
FlyBase ID	FBrf0216574
Publication Type	Research paper
PubMed ID	21923734
PubMed Abstract	Golgi-localized, γ-ear-containing, ADP ribosylation factor-binding (GGA) proteins are monomeric adaptors implicated in clathrin-mediated vesicular transport between the trans Golgi network and endosomes, characterized mainly from cell culture analysis of lysosomal sorting. To provide the first demonstration of GGA's role in vivo, we used Drosophila which has a single GGA and a single lysosomal sorting receptor, lysosomal enzyme receptor protein (LERP). Using RNAi knockdowns, we show that the Drosophila GGA is required for lysosomal sorting. We further identified authentic components of the Drosophila lysosomal sorting system--the sorting receptor LERP, the sorting adaptor GGA and the lysosomal cargo cathepsins B1, D and L--to show that GGA depletion results in lysosomal dysfunction. Abnormal lysosomal morphology, missorting of lysosomal cathepsins and impaired lysosomal proteolysis show disturbed LERP trafficking after GGA depletion. GGA is highly expressed in the mushroom bodies and the pigment cells of the retina, and increasing or decreasing the levels of GGA in the eyes leads to retinal defects. Reduced GGA levels also enhance an eye defect caused by overexpression of the autophagy-associated protein Blue cheese (Bchs), implicating GGA in autophagic processes. This shows that Drosophila provides an excellent whole-animal model to gain new insights into the function of GGA in the physiological environment of a multicellular organism.
DOI	10.1111/j.1600-0854.2011.01285.x
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Traffic
Title	Traffic
Publication Year	2000-
ISBN/ISSN	1398-9219
Data from Reference
Alleles (21)
	bchs^EP2299 cathD^GD5487 Cp1^GD5803 Cp1^KK107765 CtsB1^GD6407 CtsB1^KK102677 Gga^{dn.Scer\UAS.T:Avic\GFP} Gga^{dsRNA.Scer\UAS.1} Gga^{dsRNA.Scer\UAS.2} Gga^GD1710 Gga^{Scer\UAS.T:Avic\GFP} Hsap\GGA3^{Scer\UAS.T:Avic\GFP} Hsap\LAMP1^{Scer\UAS.T:Avic\GFP-EGFP} Scer\GAL4^Act5C.PU Scer\GAL4^da.G32 Scer\GAL4^elav-C155 Scer\GAL4^ey.PH Scer\GAL4^GMR.PF Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} Scer\GAL4^ninaE.PD Scer\GAL4^repo
Constructs (18)
	P{Act5C-GAL4.U} P{GAL4-da.G32} P{GAL4-ey.H} P{GAL4-ninaE.GMR} P{GD1710} P{GD5487} P{GD5803} P{GD6407} P{KK102677} P{KK107765} P{matα4-GAL-VP16} P{ninaE-GAL4.D} P{UAS-GFP-LAMP} P{UAS-Gga.GFP.dn} P{UAS-Gga.GFP} P{UAS-Gga.RNAi.1} P{UAS-Gga.RNAi.2} P{UAS-hGGA3.GFP}
Genes (12)
	bchs cathD cer Cp1 CtsB1 Gga Hsap\GGA3 Hsap\LAMP1 Lerp lva Scer\GAL4 T:Avic\GFP
Insertions (3)
	P{EP}bchs^EP2299 P{GAL4}repo P{GawB}elav^C155
Natural transposons (1)
	P-element