Reference Report

Reference
Citation	Depetris-Chauvin, A., Berni, J., Aranovich, E.J., Muraro, N.I., Beckwith, E.J., Ceriani, M.F. (2011). Adult-specific electrical silencing of pacemaker neurons uncouples molecular clock from circadian outputs. Curr. Biol. 21(21): 1783--1793. (Export to RIS)
FlyBase ID	FBrf0216659
Publication Type	Research paper
PubMed ID	22018542
PubMed Abstract	Circadian rhythms regulate physiology and behavior through transcriptional feedback loops of clock genes running within specific pacemaker cells. In Drosophila, molecular oscillations in the small ventral lateral neurons (sLNvs) command rhythmic behavior under free-running conditions releasing the neuropeptide PIGMENT DISPERSING FACTOR (PDF) in a circadian fashion. Electrical activity in the sLNvs is also required for behavioral rhythmicity. Yet, how temporal information is transduced into behavior remains unclear.Here we developed a new tool for temporal control of gene expression to obtain adult-restricted electrical silencing of the PDF circuit, which led to reversible behavioral arrhythmicity. Remarkably, PERIOD (PER) oscillations during the silenced phase remained unaltered, indicating that arrhythmicity is a direct consequence of the silenced activity. Accordingly, circadian axonal remodeling and PDF accumulation were severely affected during the silenced phase.Although electrical activity of the sLNvs is not a clock component, it coordinates circuit outputs leading to rhythmic behavior.
DOI	10.1016/j.cub.2011.09.027
Related Publication(s)
Note	Circadian pacemakers: how clock properties relate to their cellular properties. Taghert, 2011, Curr. Biol. 21(21): R894--R896 [FBrf0219133]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Alleles (4)
	Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^P2.4.Pdf Scer\GAL4^Pdf.Switch1a
Constructs (4)
	P{Pdf-GAL4.P2.4} P{Pdf-GS} P{UAS-Hsap\KCNJ2.EGFP} P{UAS-mCD8::GFP.L}
Genes (5)
	Hsap\KCNJ2 Mmus\Cd8a Pdf per Scer\GAL4
Natural transposons (1)
	P-element