Open Close
Reference
Citation
Bassi, Z.I., Verbrugghe, K.J., Capalbo, L., Gregory, S., Montembault, E., Glover, D.M., D'Avino, P.P. (2011). Sticky/Citron kinase maintains proper RhoA localization at the cleavage site during cytokinesis.  J. Cell Biol. 195(4): 595--603.
FlyBase ID
FBrf0216679
Publication Type
Research paper
Abstract

In many organisms, the small guanosine triphosphatase RhoA controls assembly and contraction of the actomyosin ring during cytokinesis by activating different effectors. Although the role of some RhoA effectors like formins and Rho kinase is reasonably understood, the functions of another putative effector, Citron kinase (CIT-K), are still debated. In this paper, we show that, contrary to previous models, the Drosophila melanogaster CIT-K orthologue Sticky (Sti) does not require interaction with RhoA to localize to the cleavage site. Instead, RhoA fails to form a compact ring in late cytokinesis after Sti depletion, and this function requires Sti kinase activity. Moreover, we found that the Sti Citron-Nik1 homology domain interacts with RhoA regardless of its status, indicating that Sti is not a canonical RhoA effector. Finally, Sti depletion caused an increase of phosphorylated myosin regulatory light chain at the cleavage site in late cytokinesis. We propose that Sti/CIT-K maintains correct RhoA localization at the cleavage site, which is necessary for proper RhoA activity and contractile ring dynamics.

PubMed ID
PubMed Central ID
PMC3257531 (PMC) (EuropePMC)
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Cell Biol.
    Title
    Journal of Cell Biology
    Publication Year
    1966-
    ISBN/ISSN
    0021-9525
    Data From Reference
    Aberrations (1)
    Alleles (3)
    Genes (5)
    Physical Interactions (4)
    Cell Lines (1)
    Insertions (1)
    Transgenic Constructs (2)