Reference Report

Reference
Citation	Bassi, Z.I., Verbrugghe, K.J., Capalbo, L., Gregory, S., Montembault, E., Glover, D.M., D'Avino, P.P. (2011). Sticky/Citron kinase maintains proper RhoA localization at the cleavage site during cytokinesis. J. Cell Biol. 195(4): 595--603. (Export to RIS)
FlyBase ID	FBrf0216679
Publication Type	Research paper
PubMed ID	22084308
PubMed Abstract	In many organisms, the small guanosine triphosphatase RhoA controls assembly and contraction of the actomyosin ring during cytokinesis by activating different effectors. Although the role of some RhoA effectors like formins and Rho kinase is reasonably understood, the functions of another putative effector, Citron kinase (CIT-K), are still debated. In this paper, we show that, contrary to previous models, the Drosophila melanogaster CIT-K orthologue Sticky (Sti) does not require interaction with RhoA to localize to the cleavage site. Instead, RhoA fails to form a compact ring in late cytokinesis after Sti depletion, and this function requires Sti kinase activity. Moreover, we found that the Sti Citron-Nik1 homology domain interacts with RhoA regardless of its status, indicating that Sti is not a canonical RhoA effector. Finally, Sti depletion caused an increase of phosphorylated myosin regulatory light chain at the cleavage site in late cytokinesis. We propose that Sti/CIT-K maintains correct RhoA localization at the cleavage site, which is necessary for proper RhoA activity and contractile ring dynamics.
DOI	10.1083/jcb.201105136
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (1)
	Df(3L)iro-2
Alleles (3)
	His2Av^{T:Avic\GFP-S65T} sqh^{RLC.T:Avic\GFP-S65T} sti^GS9053
Constructs (2)
	P{His2Av^{T:Avic\GFP-S65T}} P{sqh-GFP.RLC}
Genes (5)
	His2Av Rho1 sqh sti zip
Insertions (1)
	P{GSV6}sti^GS9053