Reference Report

Reference
Citation	Lee, S., Kim, S., Nahm, M., Kim, E., Kim, T.I., Yoon, J.H., Lee, S. (2011). The phosphoinositide phosphatase Sac1 is required for midline axon guidance. Mol. Cells 32(5): 477--482. (Export to RIS)
FlyBase ID	FBrf0216733
Publication Type	Research paper
PubMed ID	22042447
PubMed Abstract	Sac1 phosphoinositide (PI) phosphatases are important regulators of PtdIns(4)P turnover at the ER, Golgi, and plasma membrane (PM) and are involved in diverse cellular processes including cytoskeletal organization and vesicular trafficking. Here, we present evidence that Sac1 regulates axon guidance in the embryonic CNS of Drosophila. Sac1 is expressed on three longitudinal axon tracts that are defined by the cell adhesion molecule Fasciclin II (Fas II). Mutations in the sac1 gene cause ectopic midline crossing of Fas II-positive axon tracts. This phenotype is rescued by neuronal expression of wild-type Sac1 but not by a catalytically-inactive mutant. Finally, sac1 displays dosage-sensitive genetic interactions with mutations in the genes that encode the midline repellent Slit and its axonal receptor Robo. Taken together, our results suggest that Sac1-mediated regulation of PIs is critical for Slit/Robo-dependent axon repulsion at the CNS midline.
DOI	10.1007/s10059-011-0168-6
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Mol. Cells
Title	Molecules and Cells
Publication Year
ISBN/ISSN	1016-8478
Data from Reference
Alleles (10)
	robo² Sac1¹ Sac1² Sac1²¹⁰⁷ Sac1^G8721 Sac1^{Scer\UAS.CS-TA.T:Ivir\HA1} Sac1^{Scer\UAS.WT.T:Ivir\HA1} Scer\GAL4^da.G32 Scer\GAL4^elav-C155 sli²
Constructs (4)
	P{EP} P{GAL4-da.G32} P{UAS-Sac1.HA.CS-TA} P{UAS-Sac1.HA.WT}
Genes (7)
	Fas2 futsch robo Sac1 Scer\GAL4 sli T:Ivir\HA1
Insertions (3)
	P{EP}G4263 P{EP}Sac1^G8721 P{GawB}elav^C155
Natural transposons (1)
	P-element