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Citation
Abruzzi, K.C., Rodriguez, J., Menet, J.S., Desrochers, J., Zadina, A., Luo, W., Tkachev, S., Rosbash, M. (2011). Drosophila CLOCK target gene characterization: implications for circadian tissue-specific gene expression.  Genes Dev. 25(22): 2374--2386.
FlyBase ID
FBrf0216798
Publication Type
Research paper
Abstract
CLOCK (CLK) is a master transcriptional regulator of the circadian clock in Drosophila. To identify CLK direct target genes and address circadian transcriptional regulation in Drosophila, we performed chromatin immunoprecipitation (ChIP) tiling array assays (ChIP-chip) with a number of circadian proteins. CLK binding cycles on at least 800 sites with maximal binding in the early night. The CLK partner protein CYCLE (CYC) is on most of these sites. The CLK/CYC heterodimer is joined 4-6 h later by the transcriptional repressor PERIOD (PER), indicating that the majority of CLK targets are regulated similarly to core circadian genes. About 30% of target genes also show cycling RNA polymerase II (Pol II) binding. Many of these generate cycling RNAs despite not being documented in prior RNA cycling studies. This is due in part to different RNA isoforms and to fly head tissue heterogeneity. CLK has specific targets in different tissues, implying that important CLK partner proteins and/or mechanisms contribute to gene-specific and tissue-specific regulation.
PubMed ID
PubMed Central ID
PMC3222903 (PMC) (EuropePMC)
Related Publication(s)
Note
A master CLOCK hard at work brings rhythm to the transcriptome.
Edery, 2011, Genes Dev. 25(22): 2321--2326 [FBrf0217039]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genes Dev.
    Title
    Genes & Development
    Publication Year
    1987-
    ISBN/ISSN
    0890-9369
    Data From Reference