Reference Report

Reference
Citation	Keller, L.C., Cheng, L., Locke, C.J., Müller, M., Fetter, R.D., Davis, G.W. (2011). Glial-derived prodegenerative signaling in the Drosophila neuromuscular system. Neuron 72(5): 760--775. (Export to RIS)
FlyBase ID	FBrf0216904
Publication Type	Research paper
PubMed ID	22153373
PubMed Abstract	We provide evidence for a prodegenerative, glial-derived signaling framework in the Drosophila neuromuscular system that includes caspase and mitochondria-dependent signaling. We demonstrate that Drosophila TNF-α (eiger) is expressed in a subset of peripheral glia, and the TNF-α receptor (TNFR), Wengen, is expressed in motoneurons. NMJ degeneration caused by disruption of the spectrin/ankyrin skeleton is suppressed by an eiger mutation or by eiger knockdown within a subset of peripheral glia. Loss of wengen in motoneurons causes a similar suppression providing evidence for glial-derived prodegenerative TNF-α signaling. Neither JNK nor NFκβ is required for prodegenerative signaling. However, we provide evidence for the involvement of both an initiator and effector caspase, Dronc and Dcp-1, and mitochondrial-dependent signaling. Mutations that deplete the axon and nerve terminal of mitochondria suppress degeneration as do mutations in Drosophila Bcl-2 (debcl), a mitochondria-associated protein, and Apaf-1 (dark), which links mitochondrial signaling with caspase activity in other systems.
DOI	10.1016/j.neuron.2011.09.031
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Neuron
Title	Neuron
Publication Year	1988-
ISBN/ISSN	0896-6273
Data from Reference
Alleles (26)
	Ank2^f02001 Ark^CD4 Avic\GFP^{Scer\UAS.T:SV40\nls2} bsk^{K53R.Scer\UAS} Dcp-1^Prev1 Dcp-1^Scer\UAS.cKa Dcp-1^{Scer\UAS.T:Avic\GFP-YFP.Venus} debcl^E26 dl¹ Dredd^Scer\UAS.cKa egr^{dsRNA.Scer\UAS} egr^Δ25 Ice^Scer\UAS.cYa Miro^B682 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Nc^Scer\UAS.cKa Nc^{Scer\UAS.T:Zzzz\FLAG} Scer\GAL4^egr-GAL4 Scer\GAL4^elav-C155 Scer\GAL4^eve.RRa Scer\GAL4^repo Scer\GAL4^VGlut-OK371 T:Avic\GFP^YFP.Venus wgn^{dsRNA.Scer\UAS} wgn^{Scer\UAS.T:Avic\GFP-YFP.Venus} α-Spec^KK101541
Constructs (15)
	P{eve-GAL4.RRa} P{GAL4} P{KK101541} P{UAS-bsk.K53R} P{UAS-Dcp-1.K} P{UAS-Dcp-1.Venus} P{UAS-Dredd.K} P{UAS-eiger.IR} P{UAS-GFP.nls} P{UAS-Ice.Y} P{UAS-mCD8::GFP.L} P{UAS-Nc.FLAG} P{UAS-Nc.K} P{UAS-wengen-IR} P{UAS-wgn.Venus}
Genes (19)
	Ank2 Ark Avic\GFP brp bsk Dcp-1 debcl dl dlg1 Dredd egr Ice Miro Mmus\Cd8a Nc repo Scer\GAL4 wgn α-Spec
Insertions (5)
	P{GAL4}egr^GAL4 P{GAL4}repo P{GawB}elav^C155 P{GawB}VGlut^OK371 PBac{WH}Ank2^f02001
Natural transposons (1)
	P-element
Transcripts (1)
	Scer\GAL4^egr-GAL4RA