Reference Report

Reference
Citation	Sweeney, L.B., Chou, Y.H., Wu, Z., Joo, W., Komiyama, T., Potter, C.J., Kolodkin, A.L., Garcia, K.C., Luo, L. (2011). Secreted Semaphorins from Degenerating Larval ORN Axons Direct Adult Projection Neuron Dendrite Targeting. Neuron 72(5): 734--747. (Export to RIS)
FlyBase ID	FBrf0216932
Publication Type	Research paper
PubMed ID	22153371
PubMed Abstract	During assembly of the Drosophila olfactory circuit, projection neuron (PN) dendrites prepattern the developing antennal lobe before the arrival of axons from their presynaptic partners, the adult olfactory receptor neurons (ORNs). We previously found that levels of transmembrane Semaphorin-1a, which acts as a receptor, instruct PN dendrite targeting along the dorsolateral-ventromedial axis. Here we show that two secreted semaphorins, Sema-2a and Sema-2b, provide spatial cues for PN dendrite targeting. Sema-2a and Sema-2b proteins are distributed in gradients opposing the Sema-1a protein gradient, and Sema-1a binds to Sema-2a-expressing cells. In Sema-2a and Sema-2b double mutants, PN dendrites that normally target dorsolaterally in the antennal lobe mistarget ventromedially, phenocopying cell-autonomous Sema-1a removal from these PNs. Cell ablation, cell-specific knockdown, and rescue experiments indicate that secreted semaphorins from degenerating larval ORN axons direct dendrite targeting. Thus, a degenerating brain structure instructs the wiring of a developing circuit through the repulsive action of secreted semaphorins.
DOI	10.1016/j.neuron.2011.09.026
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Neuron
Title	Neuron
Publication Year	1988-
ISBN/ISSN	0896-6273
Data from Reference
Aberrations (1)
	Df(2R)Sema-2b-C4
Alleles (22)
	Cbβ\DT-A^I.Scer\UAS Mmus\Cd8a^Mz19 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} plexA^{Scer\UAS.T:Ivir\HA1} plexB^KG00878 plexB^Scer\UAS.cHa Scer\GAL4^elav-C155 Scer\GAL4^en-e16E Scer\GAL4^ey-OK107 Scer\GAL4^GH146 Scer\GAL4^HB5-43 Scer\GAL4^Mz19 Scer\GAL4^NP5103 Scer\GAL4^{Orco.2.642.T:Hsim\VP22} Scer\GAL4^peb-GAL4 Scer\GAL80^ts.αTub84B Sema-2a⁰³⁴⁷⁴ Sema-2a^GD5476 Sema-2a^Scer\UAS.cKa Sema-2a^{Scer\UAS.T:Avic\GFP,T:Mmus\Cd8a} Sema-2b^f02042 Sema-2b^unspecified
Constructs (10)
	P{GAL4} P{GD5476} P{Orco-GAL4.W} P{tubP-GAL80^ts} P{UAS-Cbβ\DT-A.I} P{UAS-mCD8::GFP.L} P{UAS-plexA.HA} P{UAS-plexB.H} P{UAS-Sema-2a-TM-GFP} P{UAS-sema-II.K}
Genes (11)
	brp CadN Cbβ\DT-A Mmus\Cd8a plexA plexB Scer\GAL4 Scer\GAL80 Sema-1a Sema-2a Sema-2b
Insertions (11)
	P{en2.4-GAL4}e16E P{ET-mCD8-GFP}Mz19 P{GAL4}HB5-43 P{GAL4}peb^GAL4 P{GawB}elav^C155 P{GawB}ey^OK107 P{GawB}GH146 P{GawB}Mz19 P{GawB}trol^NP5103 P{SUPor-P}plexB^KG00878 PBac{WH}Sema-2b^f02042
Transcripts (1)
	Scer\GAL4^HB5-43RA