Reference Report

Reference
Citation	Zhu, S., Barshow, S., Wildonger, J., Jan, L.Y., Jan, Y.N. (2011). Ets transcription factor Pointed promotes the generation of intermediate neural progenitors in Drosophila larval brains. Proc. Natl. Acad. Sci. U.S.A. 108(51): 20615--20620. (Export to RIS)
FlyBase ID	FBrf0216951
Publication Type	Research paper
PubMed ID	22143802
PubMed Abstract	Intermediate neural progenitor (INP) cells are transient amplifying neurogenic precursor cells generated from neural stem cells. Amplification of INPs significantly increases the number of neurons and glia produced from neural stem cells. In Drosophila larval brains, INPs are produced from type II neuroblasts (NBs, Drosophila neural stem cells), which lack the proneural protein Asense (Ase) but not from Ase-expressing type I NBs. To date, little is known about how Ase is suppressed in type II NBs and how the generation of INPs is controlled. Here we show that one isoform of the Ets transcription factor Pointed (Pnt), PntP1, is specifically expressed in type II NBs, immature INPs, and newly mature INPs in type II NB lineages. Partial loss of PntP1 in genetic mosaic clones or ectopic expression of the Pnt antagonist Yan, an Ets family transcriptional repressor, results in a reduction or elimination of INPs and ectopic expression of Ase in type II NBs. Conversely, ectopic expression of PntP1 in type I NBs suppresses Ase expression the NB and induces ectopic INP-like cells in a process that depends on the activity of the tumor suppressor Brain tumor. Our findings suggest that PntP1 is both necessary and sufficient for the suppression of Ase in type II NBs and the generation of INPs in Drosophila larval brains.
DOI	10.1073/pnas.1118595109
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (14)
	aop^Scer\UAS.cRa ase^Scer\UAS.cBa brat^HM05078 Ecol\lacZ^pnt-1277 Mmus\Cd8a^{R9D11.erm.T:Avic\GFP} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} pnt^05484Δ33 pnt^{Scer\UAS.P1.cZa} Scer\GAL4^elav-C155 Scer\GAL4^GMR9D11 Scer\GAL4^insc-Mz1407 Scer\GAL4^pnt-14-94 Scer\GAL80^ase.PN Scer\GAL80^ts.αTub84B
Constructs (10)
	P{ase-GAL80.N} P{erm-mCD8-GFP.R9D11} P{GAL4} P{GMR9D11-GAL4} P{TRiP.HM05078} P{tubP-GAL80^ts} P{UAS-aop.WT} P{UAS-ase.B} P{UAS-mCD8::GFP.L} P{UAS-pnt.P1.Z}
Genes (10)
	aop ase brat dpn Ecol\lacZ Mmus\Cd8a pnt Scer\GAL4 Scer\GAL80 T:Avic\GFP
Insertions (4)
	P{GAL4}pnt^14-94 P{GawB}elav^C155 P{GawB}insc^Mz1407 P{lacW}pnt¹²⁷⁷
Natural transposons (1)
	P-element