Reference Report

Reference
Citation	Kang, J., Kim, J., Choi, K.W. (2011). Novel Cytochrome P450, cyp6a17, Is Required for Temperature Preference Behavior in Drosophila. PLoS ONE 6(12): e29800. (Export to RIS)
FlyBase ID	FBrf0217065
Publication Type	Research paper
PubMed ID	22216356
PubMed Abstract	Perception of temperature is an important brain function for organisms to survive. Evidence suggests that temperature preference behavior (TPB) in Drosophila melanogaster, one of poikilothermal animals, is regulated by cAMP-dependent protein kinase (PKA) signaling in mushroom bodies of the brain. However, downstream targets for the PKA signaling in this behavior have not been identified. From a genome-wide search for the genes regulated by PKA activity in the mushroom bodies, we identified the cyp6a17 Cytochrome P450 gene as a new target for PKA. Our detailed analysis of mutants by genetic, molecular and behavioral assays shows that cyp6a17 is essential for temperature preference behavior. cyp6a17 expression is enriched in the mushroom bodies of the adult brain. Tissue-specific knockdown and rescue experiments demonstrate that cyp6a17 is required in the mushroom bodies for normal temperature preference behavior. This is the first study, to our knowledge, to show PKA-dependent expression of a cytochrome P450 gene in the mushroom bodies and its role as a key factor for temperature preference behavior. Taken together, this study reveals a new PKA-Cytochrome P450 pathway that regulates the temperature preference behavior.
DOI	10.1371/journal.pone.0029800
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Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS ONE
Title	PLoS ONE
Publication Year	2006-
ISBN/ISSN	1932-6203
Data from Reference
Alleles (24)
	CrebB^S162 Cyp6a17^+t4.2 Cyp6a17^{dsRNA.Scer\UAS.WIZ} Cyp6a17^KG04448 Cyp6a17^Scer\UAS.cKa Cyp6a17^Δ173 dnc¹ dnc^M14 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Mmus\Pkaca^mC.Scer\UAS Pka-R1^BDK.Scer\UAS rut¹ Scer\GAL4^Aph-4-c232 Scer\GAL4^c290 Scer\GAL4^c309 Scer\GAL4^c747 Scer\GAL4^Cyp6a17.MB Scer\GAL4^Cyp6a17-SG Scer\GAL4^elav.PU Scer\GAL4^ey-OK107 Scer\GAL4^Ilp2.215-3 Scer\GAL4^Mef2.247 Scer\GAL80^ts.αTub84B w^+mC
Constructs (12)
	P{Cyp6a17.4.2} P{Cyp6a17-GAL4.K} P{Cyp6a17-GAL4.MB} P{elav-GAL4.U} P{Ilp2-GAL4.215-3} P{Mef2-GAL4.247} P{tubP-GAL80^ts} P{UAS-Cyp6a17.K} P{UAS-Cyp6a17.WIZ} P{UAS-mCD8::GFP.L} P{UAS-PKA.mC} P{UAS-Pka-R1.BDK}
Genes (12)
	CrebB Cyp6a17 Cyp6a22 Cyp6a23 dnc Mmus\Cd8a Mmus\Pkaca Pka-R1 rut Scer\GAL4 Scer\GAL80 w
Insertions (7)
	P{Cyp6a17-GAL4.MB}SG P{GawB}Aph-4^c232 P{GawB}c290 P{GawB}c309 P{GawB}c747 P{GawB}ey^OK107 P{SUPor-P}Cyp6a17^KG04448
Natural transposons (1)
	P-element