Reference Report

Reference
Citation	Hallson, G., Hollebakken, R.E., Li, T., Syrzycka, M., Kim, I., Cotsworth, S., Fitzpatrick, K.A., Sinclair, D.A., Honda, B.M. (2012). dSet1 Is the Main H3K4 Di- and Tri-Methyltransferase Throughout Drosophila Development. Genetics 190(1): 91--100. (Export to RIS)
FlyBase ID	FBrf0217167
Publication Type	Research paper
PubMed ID	22048023
PubMed Abstract	In eukaryotes, the post-translational addition of methyl groups to histone H3 lysine 4 (H3K4) plays key roles in maintenance and establishment of appropriate gene expression patterns and chromatin states. We report here that an essential locus within chromosome 3L centric heterochromatin encodes the previously uncharacterized Drosophila melanogaster ortholog (dSet1, CG40351) of the Set1 H3K4 histone methyltransferase (HMT). Our results suggest that dSet1 acts as a "global" or general H3K4 di- and trimethyl HMT in Drosophila. Levels of H3K4 di- and trimethylation are significantly reduced in dSet1 mutants during late larval and post-larval stages, but not in animals carrying mutations in genes encoding other well-characterized H3K4 HMTs such as trr, trx, and ash1. The latter results suggest that Trr, Trx, and Ash1 may play more specific roles in regulating key cellular targets and pathways and/or act as global H3K4 HMTs earlier in development. In yeast and mammalian cells, the HMT activity of Set1 proteins is mediated through an evolutionarily conserved protein complex known as Complex of Proteins Associated with Set1 (COMPASS). We present biochemical evidence that dSet1 interacts with members of a putative Drosophila COMPASS complex and genetic evidence that these members are functionally required for H3K4 methylation. Taken together, our results suggest that dSet1 is responsible for the bulk of H3K4 di- and trimethylation throughout Drosophila development, thus providing a model system for better understanding the requirements for and functions of these modifications in metazoans.
DOI	10.1534/genetics.111.135863
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (5)
	Df(3L)1-166 Df(3L)9-56 Df(3L)K-2 Df(3L)TTT Df(3L)γ-28
Alleles (22)
	ash1^B1 ash1^B7 ash2^GD1374 ash2^{Scer\UAS.T:Ivir\HA1} Hcf^GD4353 Rbbp5^KK102877 Scer\GAL4^Act5C.PU Scer\GAL4^tub.PU Set1^G5 Set1^G12 Set1^GD4398 Set1^GD12893 Set1^Scer\UAS.cHa Set1^{Scer\UAS.T:Zzzz\FLAG} Set1^Z480 trr¹ trr^JF03242 trx^B11 trx^KK100756 trx^Z11 Wdr82^GD9340 wds^KK100120
Constructs (13)
	P{Act5C-GAL4.U} P{GD1374} P{GD4353} P{GD4398} P{GD9340} P{GD12893} P{KK100120} P{KK100756} P{KK102877} P{TRiP.JF03242} P{tub-GAL4.U} P{UAS-ash2.HA} P{UAS-Set1.H}
Genes (12)
	ash1 ash2 Hcf His3 Rbbp5 Scer\GAL4 Set1 T:Zzzz\FLAG trr trx Wdr82 wds
Natural transposons (1)
	P-element