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Chen, C.C., Wu, J.K., Lin, H.W., Pai, T.P., Fu, T.F., Wu, C.L., Tully, T., Chiang, A.S. (2012). Visualizing long-term memory formation in two neurons of the Drosophila brain.  Science 335(6069): 678--685.
FlyBase ID
FBrf0217453
Publication Type
Research paper
Abstract

Long-term memory (LTM) depends on the synthesis of new proteins. Using a temperature-sensitive ribosome-inactivating toxin to acutely inhibit protein synthesis, we screened individual neurons making new proteins after olfactory associative conditioning in Drosophila. Surprisingly, LTM was impaired after inhibiting protein synthesis in two dorsal-anterior-lateral (DAL) neurons but not in the mushroom body (MB), which is considered the adult learning and memory center. Using a photoconvertible fluorescent protein KAEDE to report de novo protein synthesis, we have directly visualized cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB)-dependent transcriptional activation of calcium/calmodulin-dependent protein kinase II and period genes in the DAL neurons after spaced but not massed training. Memory retention was impaired by blocking neural output in DAL during retrieval but not during acquisition or consolidation. These findings suggest an extra-MB memory circuit in Drosophila: LTM consolidation (MB to DAL), storage (DAL), and retrieval (DAL to MB).

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Related Publication(s)
Note

Neuroscience. Ode to the mushroom bodies.
Dubnau, 2012, Science 335(6069): 664--665 [FBrf0217604]

Mushroom-body memories: an obituary prematurely written?
Davis and Giurfa, 2012, Curr. Biol. 22(8): R272--R275 [FBrf0220175]

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Science
    Title
    Science
    Publication Year
    1895-
    ISBN/ISSN
    0036-8075
    Data From Reference
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    Natural transposons (1)
    Insertions (18)
    Experimental Tools (2)
    Transgenic Constructs (21)
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