Reference Report

Reference
Citation	Legent, K., Steinhauer, J., Richard, M., Treisman, J.E. (2012). A screen for x-linked mutations affecting Drosophila photoreceptor differentiation identifies casein kinase 1α as an essential negative regulator of wingless signaling. Genetics 190(2): 601--616. (Export to RIS)
FlyBase ID	FBrf0217484
Publication Type	Research paper
PubMed ID	22095083
PubMed Abstract	The Wnt and Hedgehog signaling pathways are essential for normal development and are misregulated in cancer. The casein kinase family of serine/threonine kinases regulates both pathways at multiple levels. However, it has been difficult to determine whether individual members of this family have distinct functions in vivo, due to their overlapping substrate specificities. In Drosophila melanogaster, photoreceptor differentiation is induced by Hedgehog and inhibited by Wingless, providing a sensitive system in which to identify regulators of each pathway. We used a mosaic genetic screen in the Drosophila eye to identify mutations in genes on the X chromosome required for signal transduction. We recovered mutations affecting the transcriptional regulator CREB binding protein, the small GTPase dynamin, the cytoskeletal regulator Actin-related protein 2, and the protein kinase Casein kinase 1α. Consistent with its reported function in the β-Catenin degradation complex, Casein Kinase 1α mutant cells accumulate β-Catenin and ectopically induce Wingless target genes. In contrast to previous studies based on RNA interference, we could not detect any effect of the same Casein Kinase 1α mutation on Hedgehog signaling. We thus propose that Casein kinase 1α is essential to allow β-Catenin degradation and prevent inappropriate Wingless signaling, but its effects on the Hedgehog pathway are redundant with other Casein kinase 1 family members.
DOI	10.1534/genetics.111.133827
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (4)
	Df(1)19 Dp(1;4)r⁺l Dp(1;Y)shi⁺3 Dp(1;Y)W73
Alleles (27)
	3B1^3B1 4A1^4A1 8D2^8D2 Arp2^casa CG2577^{Scer\UAS.T:Zzzz\FLAG} CkIα^8B12 CkIα^{Scer\UAS.T:Zzzz\FLAG} csw^8C7 dco³ dco^KD.Scer\UAS Disc\RFP^{fz3.T:nls-tra} dlg1^6C2 Ecol\lacZ^aos-W11 Ecol\lacZ^Dll-01092 Ecol\lacZ^dpp.BS3.0 Ecol\lacZ^ds-05142 Ecol\lacZ^ptc-AT90 fw^1B2 lz^4B4 nej^8B27 pan^ΔN.Scer\UAS rap^7C2 Scer\GAL4^ptc-559.1 Scer\GAL4^tub.PU sgg^3B5 sgg^8C18 shi^7C7
Constructs (7)
	P{BS3.0} P{fz3-RFP.O} P{tub-GAL4.U} P{UAS-CG2577.FLAG} P{UAS-CkIα.FLAG} P{UAS-dco.KD} P{UAS-pan.dTCFΔN}
Genes (42)
	3B1 4A1 8D2 aos arm Arp2 ato bi CG2577 ci CkIα csw dco Disc\RFP Dl dlg1 Dll dm dpp ds Dsor1 E(spl)m8-HLH Ecol\lacZ Egfr elav en fw fz3 lz N nej pan phl ptc rap Scer\GAL4 sens sgg shi smo vg wg
Insertions (8)
	P{EPgy2}Axs^EY00887 P{EPgy2}CanA-14F^EY08594 P{EPgy2}Rbp2^EY00852 P{GawB}ptc^559.1 P{lacZ-un2}ptc-AT90 P{lwB}aos^W11 P{PZ}Dll⁰¹⁰⁹² P{PZ}ds⁰⁵¹⁴²
Natural transposons (1)
	P-element