Reference Report

Reference
Citation	Zhong, L., Bellemer, A., Yan, H., Honjo, K., Robertson, J., Hwang, R.Y., Pitt, G.S., Tracey, W.D. (2012). Thermosensory and non-thermosensory isoforms of Drosophila melanogaster TRPA1 reveal heat sensor domains of a thermoTRP channel. Cell Rep. 1(1): 43--55. (Export to RIS)
FlyBase ID	FBrf0217493
Publication Type	Research paper
PubMed ID	22347718
PubMed Abstract	Specialized somatosensory neurons detect temperatures ranging from pleasantly cool or warm to burning hot and painful (nociceptive). The precise temperature ranges sensed by thermally sensitive neurons is determined by tissue-specific expression of ion channels of the transient receptor potential(TRP) family.We show here that in Drosophila, TRPA1 is required for the sensing of nociceptive heat. We identify two previously unidentified protein isoforms of dTRPA1, named dTRPA1-C and dTRPA1-D, that explain this requirement. A dTRPA1-C/D reporter was exclusively expressed in nociceptors, and dTRPA1-C rescued thermal nociception phenotypes when restored to mutant nociceptors. However,surprisingly, we find that dTRPA1-C is not a direct heat sensor. Alternative splicing generates at least four isoforms of dTRPA1. Our analysis of these isoforms reveals a 37-amino-acid-long intracellular region (encoded by a single exon) that is critical for dTRPA1 temperature responses. The identification of these amino acids opens the door to a biophysical understanding of a molecular thermosensor.
DOI	10.1016/j.celrep.2011.11.002
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Secondary IDs	FBrf0217572
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Cell Rep.
Title	Cell reports
Publication Year
ISBN/ISSN	2211-1247
Data from Reference
Aberrations (1)
	Df(3L)ED4415
Alleles (10)
	Dcr-2^Scer\UAS.cDa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^ppk.1.9 Scer\GAL4^TrpA1.C-D Scer\GAL4^TrpA1.SH TrpA1^+t21 TrpA1^C.Scer\UAS TrpA1^GD2375 TrpA1^Scer\UAS.cKa TrpA1^W903
Constructs (9)
	P{GD2375} P{ppk-GAL4.1.9} P{TrpA1^+t21} P{TrpA1-GAL4.C-D} P{TrpA1-GAL4.SH} P{UAS-Dcr-2.D} P{UAS-mCD8::GFP.L} P{UAS-TrpA1.C} P{UAS-TrpA1.K}
Genes (4)
	Dcr-2 Mmus\Cd8a Scer\GAL4 TrpA1
Natural transposons (1)
	P-element