Reference Report

Reference
Citation	Chen, X., Ganetzky, B. (2012). A neuropeptide signaling pathway regulates synaptic growth in Drosophila. J. Cell Biol. 196(4): 529--543. (Export to RIS)
FlyBase ID	FBrf0217500
Publication Type	Research paper
PubMed ID	22331845
PubMed Abstract	Neuropeptide signaling is integral to many aspects of neural communication, particularly modulation of membrane excitability and synaptic transmission. However, neuropeptides have not been clearly implicated in synaptic growth and development. Here, we demonstrate that cholecystokinin-like receptor (CCKLR) and drosulfakinin (DSK), its predicted ligand, are strong positive growth regulators of the Drosophila melanogaster larval neuromuscular junction (NMJ). Mutations of CCKLR or dsk produced severe NMJ undergrowth, whereas overexpression of CCKLR caused overgrowth. Presynaptic expression of CCKLR was necessary and sufficient for regulating NMJ growth. CCKLR and dsk mutants also reduced synaptic function in parallel with decreased NMJ size. Analysis of double mutants revealed that DSK/CCKLR regulation of NMJ growth occurs through the cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA)-cAMP response element binding protein (CREB) pathway. Our results demonstrate a novel role for neuropeptide signaling in synaptic development. Moreover, because the cAMP-PKA-CREB pathway is required for structural synaptic plasticity in learning and memory, DSK/CCKLR signaling may also contribute to these mechanisms.
DOI	10.1083/jcb.201109044
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (9)
	Df(1)ED7413 Df(1)Exel9051 Df(1)fu-A7 Df(1)os^UE69 Df(2L)N22-14 Df(3R)2-2 Dp(1;3)DC351 Dp(1;3)DC352 Dp(3;1)2-2
Alleles (26)
	CCKLR-17D1⁸⁸ CCKLR-17D1^GD17263 CCKLR-17D1^MB02688 CCKLR-17D1^Scer\UAS.cCa CCKLR-17D1^{Scer\UAS.T:Disc\RFP-mCherry} CCKLR-17D3^KG01373 CrebB^cor.Scer\UAS CrebB^DN.Scer\UAS CrebB^EP1378 CrebB^S162 Dcr-2^Scer\UAS.cDa Dsk^EP3632 Dsk^f02468b Dsk^GD6123 Dsk^Scer\UAS.cCa Gαs^{Q215L.Scer\UAS} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Pka-C1^BG02142 rut¹ Scer\GAL4^B185 Scer\GAL4^c739 Scer\GAL4^elav.PLu Scer\GAL4^elav-C155 Scer\GAL4^how-24B Scer\GAL4^Rapgap1-OK6 T:Disc\RFP^mCherry
Constructs (12)
	P{GAL4-elav.L} P{GD6123} P{GD17263} P{UAS-CCKLR-17D1.C} P{UAS-CCKLR-17D1.mCherry} P{UAS-CrebB-17A.DN} P{UAS-CrebB-17A-a.cor} P{UAS-Dcr-2.D} P{UAS-Dsk.C} P{UAS-G-sα60A.Q215L} P{UAS-mCD8::GFP.L} PBac{WH}
Genes (14)
	brp CCKLR-17D1 CCKLR-17D3 CrebB Dcr-2 Dsk elav GluRIIB GluRIIC Gαs Mmus\Cd8a Pka-C1 rut Scer\GAL4
Insertions (11)
	Mi{ET1}CCKLR-17D1^MB02688 P{EP}CrebB^EP1378 P{EP}EP3632 P{GawB}B185 P{GawB}elav^C155 P{GawB}how^24B P{GawB}Hr39^c739 P{GawB}OK6 P{GT1}Pka-C1^BG02142 P{SUPor-P}CCKLR-17D3^KG01373 PBac{WH}Dsk^f02468b
Natural transposons (1)
	P-element