|Citation||Rudolf, A., Buttgereit, D., Rexer, K.H., Renkawitz-Pohl, R. (2012). The syncytial visceral and somatic musculature develops independently of β3-Tubulin during Drosophila embryogenesis, while maternally supplied β1-Tubulin is stable until the early steps of myoblast fusion. Europ. J. Cell Biol. 91(3): 192--203. (Export to RIS)|
|Publication Type||Research paper|
|PubMed Abstract||Microtubules are necessary for fusion and elongation of vertebrate muscle cells. In Drosophila, several isoforms of β-Tubulin, the functional subunit of microtubules, are expressed in different tissues of the developing embryo, while solely the β3-Tubulin isoform is detected in large amounts during differentiation of the somatic and visceral musculature. Here we show the unexpected result that all mesodermal tissues develop correctly in β3-Tubulin loss of function mutants. Furthermore, we show that β2-Tubulin transcripts are not detectable in embryos and an exceptional zygotic β1-Tubulin expression in β3-Tubulin mutants cannot be observed. Nevertheless, a maternally contributed β1-Tubulin-GFP fusion protein (from protein trap collection, Buszczak et al., 2007, Genetics 175, 1505-1531) acts in a dominant negative way, disturbing embryonic development from early stages on. This effect can be observed to the same extent in a zygotic β3-Tubulin mutant situation. Our results indicate that the maternally supplied β1-Tubulin based microtubule network is sufficient for myoblast fusion, myotube elongation and sarcomere formation both during visceral and somatic muscle development in Drosophila embryogenesis.|
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|Language of Publication||English|
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|Also Published As|
|Abbreviation||Europ. J. Cell Biol.|
|Title||European Journal of Cell Biology|
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|Natural transposons (1)|