Reference Report

Reference
Citation	Rudolf, A., Buttgereit, D., Rexer, K.H., Renkawitz-Pohl, R. (2012). The syncytial visceral and somatic musculature develops independently of β3-Tubulin during Drosophila embryogenesis, while maternally supplied β1-Tubulin is stable until the early steps of myoblast fusion. Europ. J. Cell Biol. 91(3): 192--203. (Export to RIS)
FlyBase ID	FBrf0217503
Publication Type	Research paper
PubMed ID	22306378
PubMed Abstract	Microtubules are necessary for fusion and elongation of vertebrate muscle cells. In Drosophila, several isoforms of β-Tubulin, the functional subunit of microtubules, are expressed in different tissues of the developing embryo, while solely the β3-Tubulin isoform is detected in large amounts during differentiation of the somatic and visceral musculature. Here we show the unexpected result that all mesodermal tissues develop correctly in β3-Tubulin loss of function mutants. Furthermore, we show that β2-Tubulin transcripts are not detectable in embryos and an exceptional zygotic β1-Tubulin expression in β3-Tubulin mutants cannot be observed. Nevertheless, a maternally contributed β1-Tubulin-GFP fusion protein (from protein trap collection, Buszczak et al., 2007, Genetics 175, 1505-1531) acts in a dominant negative way, disturbing embryonic development from early stages on. This effect can be observed to the same extent in a zygotic β3-Tubulin mutant situation. Our results indicate that the maternally supplied β1-Tubulin based microtubule network is sufficient for myoblast fusion, myotube elongation and sarcomere formation both during visceral and somatic muscle development in Drosophila embryogenesis.
DOI	10.1016/j.ejcb.2011.11.002
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Europ. J. Cell Biol.
Title	European Journal of Cell Biology
Publication Year	1979-
ISBN/ISSN	0171-9335
Data from Reference
Aberrations (2)
	Df(2R)Exel6082 Df(2R)Px2
Alleles (7)
	Avic\GFP^{βTub56D-CC02069} Ecol\lacZ^kirre-rP298 Scer\GAL4^Act.PU Scer\GAL4^how-24B βTub56D^CC02069 βTub60D² βTub60D^{Scer\UAS.T:Hsap\MYC}
Constructs (2)
	M{UAS-βTub60D.10xMyc} P{Act-GAL4.U}
Genes (12)
	Avic\GFP CG10641 Ecol\lacZ Fas3 kirre Mef2 Scer\GAL4 T:Hsap\MYC Tm1 βTub56D βTub60D βTub85D
Insertions (3)
	P{GawB}how^24B P{PTT-GC}βTub56D^CC02069 P{PZ}rP298
Natural transposons (1)
	Dmau\mariner