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Citation
Anand, A., Kai, T. (2012). The tudor domain protein Kumo is required to assemble the nuage and to generate germline piRNAs in Drosophila.  EMBO J. 31(4): 870--882.
FlyBase ID
FBrf0217541
Publication Type
Research paper
Abstract

In Drosophila ovaries, distinct Piwi-interacting RNA (piRNA) pathways defend against transposons in somatic and germline cells. Germline piRNAs predominantly arise from bidirectional clusters and are amplified by the ping-pong cycle. In this study, we characterize a novel Drosophila gene, kumo and show that it encodes a conserved germline piRNA pathway component. Kumo contains five tudor domains and localizes to nuage, a unique structure present in animal germline cells, which is considered to be the processing site for germline piRNAs. Transposons targeted by the germline piRNA pathway are derepressed in kumo mutant females. Moreover, germline piRNA production is significantly reduced in mutant ovaries, thereby indicating that kumo is required to generate germline piRNAs. Kumo localizes to the nuage as well as to nucleus early female germ cells, where it is required to maintain cluster transcript levels. Our data suggest that kumo facilitates germline piRNA production by promoting piRNA cluster transcription in the nucleus and piRNA processing at the nuage.

PubMed ID
PubMed Central ID
PMC3280549 (PMC) (EuropePMC)
Related Publication(s)
Research paper

Antisense piRNA amplification, but not piRNA production or nuage assembly, requires the Tudor-domain protein Qin.
Zhang et al., 2014, EMBO J. 33(6): 536--539 [FBrf0225029]

Note

Response to Zhang et al.
Anand and Kai, 2014, EMBO J. 33(6): 540--541 [FBrf0225104]

Associated Information
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Associated Files
Other Information
Secondary IDs
  • FBrf0217469
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
EMBO J.
Title
The EMBO Journal
Publication Year
1982-
ISBN/ISSN
0261-4189
Data From Reference
Aberrations (1)
Alleles (16)
Genes (19)
Physical Interactions (9)
Cell Lines (1)
Natural transposons (17)
Insertions (2)
Experimental Tools (2)
Transgenic Constructs (4)