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Citation
Mariappa, D., Sauert, K., Marino, K., Turnock, D., Webster, R., van Aalten, D.M.F., Ferguson, M.A.J., Muller, H.A.J. (2011). Protein O-GlcNAcylation Is Required for Fibroblast Growth Factor Signaling in Drosophila.  Sci. Signal. 4(204): ra89.
FlyBase ID
FBrf0217579
Publication Type
Research paper
Abstract

Glycosylation is essential for growth factor signaling through N-glycosylation of ligands and receptors and the biosynthesis of proteoglycans as co-receptors. Here, we show that protein O-GlcNAcylation is crucial for fibroblast growth factor (FGF) signaling in Drosophila. We found that nesthocker (nst) encodes a phosphoacetylglucosamine mutase and that nst mutant embryos exhibited low amounts of intracellular uridine 5'-diphosphate-N-acetylglucosamine (UDP-GlcNAc), which disrupted protein O-GlcNAcylation. Nst was required for mitogen-activated protein kinase (MAPK) signaling downstream of FGF but not MAPK signaling activated by epidermal growth factor. nst was dispensable for the function of the FGF ligands and the FGF receptor's extracellular domain but was essential in the signal-receiving cells downstream of the FGF receptor. We identified the adaptor protein Downstream of FGF receptor (Dof), which interacts with the FGF receptor, as the relevant target for O-GlcNAcylation in the FGF pathway, suggesting that protein O-GlcNAcylation of the activated receptor complex is essential for FGF signal transduction.

PubMed ID
PubMed Central ID
PMC3660836 (PMC) (EuropePMC)
Related Publication(s)
Note

A Sweet Spot in the FGFR Signal Transduction Pathway.
Ghabrial, 2012, Sci. Signal. 5(207): pe1 [FBrf0217188]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Sci. Signal.
    Title
    Science signaling
    ISBN/ISSN
    1937-9145 1945-0877
    Data From Reference