Reference Report

Reference
Citation	Mariappa, D., Sauert, K., Marino, K., Turnock, D., Webster, R., van Aalten, D.M.F., Ferguson, M.A.J., Muller, H.A.J. (2011). Protein O-GlcNAcylation Is Required for Fibroblast Growth Factor Signaling in Drosophila. Sci. Signal. 4(204): ra89. (Export to RIS)
FlyBase ID	FBrf0217579
Publication Type	Research paper
PubMed ID	22375049
PubMed Abstract	Glycosylation is essential for growth factor signaling through N-glycosylation of ligands and receptors and the biosynthesis of proteoglycans as co-receptors. Here, we show that protein O-GlcNAcylation is crucial for fibroblast growth factor (FGF) signaling in Drosophila. We found that nesthocker (nst) encodes a phosphoacetylglucosamine mutase and that nst mutant embryos exhibited low amounts of intracellular uridine 5'-diphosphate-N-acetylglucosamine (UDP-GlcNAc), which disrupted protein O-GlcNAcylation. Nst was required for mitogen-activated protein kinase (MAPK) signaling downstream of FGF but not MAPK signaling activated by epidermal growth factor. nst was dispensable for the function of the FGF ligands and the FGF receptor's extracellular domain but was essential in the signal-receiving cells downstream of the FGF receptor. We identified the adaptor protein Downstream of FGF receptor (Dof), which interacts with the FGF receptor, as the relevant target for O-GlcNAcylation in the FGF pathway, suggesting that protein O-GlcNAcylation of the activated receptor complex is essential for FGF signal transduction.
DOI	10.1126/scisignal.2002335
Related Publication(s)
Note	A Sweet Spot in the FGFR Signal Transduction Pathway. Ghabrial, 2012, Sci. Signal. 5(207): pe1 [FBrf0217188]
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Sci. Signal.
Title	Science signaling
Publication Year
ISBN/ISSN	1937-9145
Data from Reference
Aberrations (1)
	Df(3L)ED4486
Alleles (21)
	Avic\GFP^{Scer\UAS.T:Ocun\LPH-ss,T:Hsap\LFA3-GPI} btl::htl^BH.Scer\UAS btl^dev1 htl::tor^Scer\UAS.cDa htl^AB42 htl^{Scer\UAS.T:λ\cI-DD} htl^YY262 mmy^IK63 nst¹⁶⁹²³ nst^c04986 nst^hsp.PM nst^{S86A.Scer\UAS.T:Ivir\HA1} nst^{Scer\UAS.T:Ivir\HA1} Oga^{Scer\UAS.T:Ivir\HA1} Scer\GAL4^Act.PU Scer\GAL4^btl.PS Scer\GAL4^twi.PU sxc¹ sxc⁶ sxc^{Scer\UAS.T:Ivir\HA1} wg^CE7
Constructs (12)
	P{Act-GAL4.U} P{GAL4-btl.S} P{hs-nst.M} P{twi-GAL4.U} P{UAS-btl::htl.BH} P{UAS-GFP.gpi} P{UAS-htl::tor.D} P{UAS-htl.λ\cI.M} P{UAS-nst.HA} P{UAS-nst.S86A} P{UAS-Oga.HA} P{UAS-sxc.HA}
Genes (18)
	Avic\GFP btl btl::htl en eve htl htl::tor mmy nst Oga rl Scer\GAL4 stumps sxc T:Ivir\HA1 twi verm wg
Insertions (1)
	PBac{PB}nst^c04986
Natural transposons (1)
	P-element