Reference Report

Reference
Citation	Lutas, A., Wahlmark, C.J., Acharjee, S., Kawasaki, F. (2012). Genetic analysis in Drosophila reveals a role for the mitochondrial protein p32 in synaptic transmission. G3 (Bethesda) 2(1): 59--69. (Export to RIS)
FlyBase ID	FBrf0217628
Publication Type	Research paper
PubMed ID	22384382
PubMed Abstract	Mitochondria located within neuronal presynaptic terminals have been shown to play important roles in the release of chemical neurotransmitters. In the present study, a genetic screen for synaptic transmission mutants of Drosophila has identified the first mutation in a Drosophila homolog of the mitochondrial protein P32. Although P32 is highly conserved and has been studied extensively, its physiological role in mitochondria remains unknown and it has not previously been implicated in neural function. The Drosophila P32 mutant, referred to as dp32(EC1), exhibited a temperature-sensitive (TS) paralytic behavioral phenotype. Moreover, electrophysiological analysis at adult neuromuscular synapses revealed a TS reduction in the amplitude of excitatory postsynaptic currents (EPSC) and indicated that dP32 functions in neurotransmitter release. These studies are the first to address P32 function in Drosophila and expand our knowledge of mitochondrial proteins contributing to synaptic transmission.
DOI	10.1534/g3.111.001586
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	G3 (Bethesda)
Title	G3 : genes - genomes - genetics
Publication Year
ISBN/ISSN	2160-1836
Data from Reference
Aberrations (4)
	Df(2R)14F06W-06 Df(2R)14F06W-07 Df(2R)14F06W-08 Df(2R)14F06W-09
Alleles (13)
	Avic\GFP^{Cameleon3.6.Scer\UAS} Avic\GFP^{GCaMP3.20xScer\UAS} Avic\GFP^{GCaMP3.Scer\UAS.mito} Avic\GFP^{S65T.Scer\UAS.T:Ivir\HA1,T:Hsap\mito} cac^TS2 Disc\RFP^{Scer\UAS.T:Hsap\mito} Mmus\Cd8a^{Scer\UAS.T:Disc\RFP-mRFP} P32^EC1 P32^Scer\UAS.cLa P32^{Scer\UAS.T:Avic\GFP-EGFP} Scer\GAL4^Appl.G1a Scer\GAL4^elav.PU T:Avic\GFP^EGFP
Constructs (10)
	P{20XUAS-GCaMP3} P{Appl-GAL4.G1a} P{elav-GAL4.U} P{UAS-Cameleon.3.6} P{UAS-DsRed.mito} P{UAS-GCaMP3.mito} P{UAS-mCD8.mRFP} P{UAS-mito-HA-GFP.AP} P{UAS-P32.EGFP} P{UAS-P32.L}
Genes (13)
	Avic\GFP brp cac Disc\RFP Mmus\Cd8a P32 Scer\GAL4 Syt1 T:Ggal\MLCK T:Hsap\mito T:Ocun\Mlck2 T:Rat\Cam T:Xlae\Cam
Natural transposons (1)
	P-element