Reference Report

Reference
Citation	Gault, W.J., Olguin, P., Weber, U., Mlodzik, M. (2012). Drosophila CK1-γ, gilgamesh, controls PCP-mediated morphogenesis through regulation of vesicle trafficking. J. Cell Biol. 196(5): 605--621. (Export to RIS)
FlyBase ID	FBrf0217630
Publication Type	Research paper
PubMed ID	22391037
PubMed Abstract	Cellular morphogenesis, including polarized outgrowth, promotes tissue shape and function. Polarized vesicle trafficking has emerged as a fundamental mechanism by which protein and membrane can be targeted to discrete subcellular domains to promote localized protrusions. Frizzled (Fz)/planar cell polarity (PCP) signaling orchestrates cytoskeletal polarization and drives morphogenetic changes in such contexts as the vertebrate body axis and external Drosophila melanogaster tissues. Although regulation of Fz/PCP signaling via vesicle trafficking has been identified, the interplay between the vesicle trafficking machinery and downstream terminal PCP-directed processes is less established. In this paper, we show that Drosophila CK1-γ/gilgamesh (gish) regulates the PCP-associated process of trichome formation through effects on Rab11-mediated vesicle recycling. Although the core Fz/PCP proteins dictate prehair formation broadly, CK1-γ/gish restricts nucleation to a single site. Moreover, CK1-γ/gish works in parallel with the Fz/PCP effector multiple wing hairs, which restricts prehair formation along the perpendicular axis to Gish. Our findings suggest that polarized Rab11-mediated vesicle trafficking regulated by CK1-γ is required for PCP-directed processes.
DOI	10.1083/jcb.201107137
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Alleles (46)
	Avic\GFP^Scer\UAS.cUa Dcr-2^Scer\UAS.cDa fz²¹ fz^{arm.T:Avic\GFP-EGFP} gish^{D187N.Scer\UAS.T:Hsap\MYC} gish^{dsRNA.Scer\UAS} gish^e01759 gish^GD10588 gish^{Scer\UAS.RB.T:Hsap\MYC} gish^{Scer\UAS.RF.T:Hsap\MYC} gish^Spider Hem^{Scer\UAS.T:Myr-Src64B} Mmus\Cd8a^{Scer\UAS.T:Disc\RFP-mCherry} mwh¹ mwh^GD8165 mwh^Scer\UAS.cYa nuf^VDRC.cUa Pten^GD13500 Rab4^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} Rab4^{S22N.Scer\UAS.P\T.T:Avic\GFP-YFP} Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} Rab7^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} Rab7^{T22N.Scer\UAS.P\T.T:Avic\GFP-YFP} Rab11^{dsRNA.Scer\UAS.WIZ} Rab11^GD11761 Rab11^{Q70L.Scer\UAS.P\T.T:Avic\GFP-YFP} Rab11^{S25N.Scer\UAS.P\T.T:Avic\GFP-YFP} Rab11^{Scer\UAS.P\T.T:Avic\GFP-YFP} rok² SCAR^Δ37 Scer\FLP1^hs.PS Scer\GAL4^Act5C.PI Scer\GAL4^dpp.blk1 Scer\GAL4^en-e16E Scer\GAL4^nub.PU Scer\GAL4^pnr-MD237 Scer\GAL4^{Scer\FRT.Act5C} Scer\GAL4^sev.PU Scer\GAL80^ts.αTub84B sec15^{C.Scer\UAS.T:Avic\GFP} sec15^KK101708 sqh^{E20E21.Scer\UAS.cCa} Vang^{Act5C.T:Avic\GFP-YFP} WASp³ zip¹
Constructs (36)
	P{Act5C-GAL4} P{Act5C-Vang.YFP} P{arm-fz.GFP} P{AyGAL4} P{GAL4-dpp.blk1} P{GD8165} P{GD10588} P{GD11761} P{GD13500} P{hsp70-flp} P{KK101708} P{nub-GAL4.U} P{sev-GAL4.U} P{tubP-GAL80^ts} P{UAS-Dcr-2.D} P{UAS-GFP.U} P{UAS-gish.IR2} P{UAS-gish.KD.myc} P{UAS-gish.RB.myc} P{UAS-gish.RF.myc} P{UAS-Hem.Myr} P{UAS-mCD8.ChRFP} P{UAS-mwh.Y} P{UASp-YFP.Rab4.Q67L} P{UASp-YFP.Rab4.S22N} P{UASp-YFP.Rab5.Q88L} P{UASp-YFP.Rab5.S43N} P{UASp-YFP.Rab7.Q67L} P{UASp-YFP.Rab7.T22N} P{UASp-YFP.Rab11.Q70L} P{UASp-YFP.Rab11.S25N} P{UASp-YFP.Rab11} P{UAS-Rab11.dsRNA.WIZ} P{UAS-sec15.GFP} P{UAS-sqh.E20E21.C} P{VDRC.nuf.U}
Genes (31)
	Avic\GFP Dcr-2 didum en fz gish Hem Hrs lva Mmus\Cd8a mwh nuf Pten Rab4 Rab5 Rab7 Rab11 Rip11 rok SCAR Scer\FLP1 Scer\GAL4 Scer\GAL80 sec5 sec15 sqh stan T:Hsap\MYC Vang WASp zip
Insertions (4)
	P{en2.4-GAL4}e16E P{GawB}pnr^MD237 P{PTT-GB}gish^Spider PBac{RB}gish^e01759
Natural transposons (1)
	P-element