Open Close
Reference
Citation
Neumüller, R.A., Wirtz-Peitz, F., Lee, S., Kwon, Y., Buckner, M., Hoskins, R.A., Venken, K.J., Bellen, H.J., Mohr, S.E., Perrimon, N. (2012). Stringent analysis of gene function and protein-protein interactions using fluorescently tagged genes.  Genetics 190(3): 931--940.
FlyBase ID
FBrf0217811
Publication Type
Research paper
Abstract

In Drosophila collections of green fluorescent protein (GFP) trap lines have been used to probe the endogenous expression patterns of trapped genes or the subcellular localization of their protein products. Here, we describe a method, based on nonoverlapping, highly specific, shRNA transgenes directed against GFP, that extends the utility of these collections to loss-of-function studies. Furthermore, we used a MiMIC transposon to generate GFP traps in Drosophila cell lines with distinct subcellular localization patterns, which will permit high-throughput screens using fluorescently tagged proteins. Finally, we show that fluorescent traps, paired with recombinant nanobodies and mass spectrometry, allow the study of endogenous protein complexes in Drosophila.

PubMed ID
PubMed Central ID
PMC3296255 (PMC) (EuropePMC)
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference