Reference Report

Reference
Citation	Deluca, S.Z., O'Farrell, P.H. (2012). Barriers to male transmission of mitochondrial DNA in sperm development. Dev. Cell 22(3): 660--668. (Export to RIS)
FlyBase ID	FBrf0217815
Publication Type	Research paper
PubMed ID	22421049
PubMed Abstract	Across the eukaryotic phylogeny, offspring usually inherit their mitochondrial genome from only one of two parents: in animals, the female. Although mechanisms that eliminate paternally derived mitochondria from the zygote have been sought, the developmental stage at which paternal transmission of mitochondrial DNA is restricted is unknown in most animals. Here, we show that the mitochondria of mature Drosophila sperm lack DNA, and we uncover two processes that eliminate mitochondrial DNA during spermatogenesis. Visualization of mitochondrial DNA nucleoids revealed their abrupt disappearance from developing spermatids in a process requiring the mitochondrial nuclease, Endonuclease G. In Endonuclease G mutants, persisting nucleoids are swept out of spermatids by a cellular remodeling process that trims and shapes spermatid tails. Our results show that mitochondrial DNA is eliminated during spermatogenesis, thereby removing the capacity of sperm to transmit the mitochondrial genome to the next generation.
DOI	10.1016/j.devcel.2011.12.021
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
ISBN/ISSN
Data from Reference
Aberrations (4)
	Df(2L)TENGL1-3 Df(2R)BSC699 Df(3R)TENGL4¹ Df(3R)TENGL4²
Alleles (7)
	Avic\GFP^{EYFP.sqh.T:Hsap\mito} dj^{T:Avic\GFP-S65T} EndoG^MB07150 EndoG^{Scer\UAS.P\T.T:Hsap\MYC} EndoGI^e00915 mt:ND2^del1 Scer\GAL4^{nos.UTR.T:Hsim\VP16}
Constructs (4)
	P{dj-GFP.S} P{GAL4::VP16-nos.UTR} P{sqh-EYFP-Mito} P{UASp-EndoG.MYC}
Genes (12)
	Avic\GFP CG34114 dj EndoG EndoGI mt:ND2 Scer\GAL4 T:Hsap\MYC Tengl1 Tengl2 Tengl3 Tengl4
Insertions (7)
	Mi{ET1}EndoG^MB07150 P{XP}CG4674^d00504 P{XP}d02058 P{XP}d03282 PBac{WH}f03102 PBac{WH}f07198 PBac{WH}Tengl3^f06847
Natural transposons (1)
	P-element