Reference Report

Reference
Citation	Schottenfeld-Roames, J., Ghabrial, A.S. (2012). Whacked and Rab35 polarize dynein-motor-complex-dependent seamless tube growth. Nat. Cell Biol. 14(4): 386--393. (Export to RIS)
FlyBase ID	FBrf0217936
Publication Type	Research paper
PubMed ID	22407366
PubMed Abstract	Seamless tubes form intracellularly without cell-cell or autocellular junctions. Such tubes have been described across phyla, but remain mysterious despite their simple architecture. In Drosophila, seamless tubes are found within tracheal terminal cells, which have dozens of branched protrusions extending hundreds of micrometres. We find that mutations in multiple components of the dynein motor complex block seamless tube growth, raising the possibility that the lumenal membrane forms through minus-end-directed transport of apical membrane components along microtubules. Growth of seamless tubes is polarized along the proximodistal axis by Rab35 and its apical membrane-localized GAP, Whacked. Strikingly, loss of whacked (or constitutive activation of Rab35) leads to tube overgrowth at terminal cell branch tips, whereas overexpression of Whacked (or dominant-negative Rab35) causes formation of ectopic tubes surrounding the terminal cell nucleus. Thus, vesicle trafficking has key roles in making and shaping seamless tubes.
DOI	10.1038/ncb2454
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Cell Biol.
Title	Nature Cell Biology
Publication Year	1999-
ISBN/ISSN	1465-7392 1476-4679
Data from Reference
Aberrations (3)
	Df(1)C128 Df(3R)Exel6276 Df(3R)M-Kx1
Alleles (29)
	Act5C^{Scer\UAS.P\T.T:Disc\RFP-mRFP} Act5C^{Scer\UAS.T:Avic\GFP} btl^{Scer\UAS.T:λ\cI-DD} crb^{KI.A.T:Avic\GFP} Dhc64C^4-19 Disc\RFP^Scer\UAS.cGb Dlic¹ Dlic² Eb1^{Scer\UAS.T:Avic\GFP-EGFP} egl¹ Gl¹ Lis-1^G10.14 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Rab35^{Q67L.Scer\UAS.P\T.T:Avic\GFP-YFP} Rab35^{S22N.Scer\UAS.T:Avic\GFP-YFP} Rab35^{Scer\UAS.P\T.T:Avic\GFP-YFP} Scer\GAL4^bs.PM Scer\GAL4^btl.PS sn^36a sn^X2 T:Zzzz\eqFP578^mKate2 T:Zzzz\eqFP578^mKate wkd²²⁰ wkd^+t3.9 wkd^GD11617 wkd^MB04624 wkd^PC24 wkd^Scer\UAS.cSa wkd^{Scer\UAS.T:Zzzz\eqFP578-mKate2}
Constructs (15)
	P{bs-GAL4.M} P{GAL4-btl.S} P{GD11617} P{UAS-Act5C.T:GFP} P{UAS-btl.λ} P{UAS-DsRed.Gb} P{UAS-Eb1.GFP} P{UAS-mCD8::GFP.L} P{UASp-Act5C.mRFP} P{UASp-YFP.Rab35.Q67L} P{UASp-YFP.Rab35} P{UAST-YFP.Rab35.S22N} P{UAS-wkd.mKate2} P{UAS-wkd.S} P{wkd3.9}
Genes (17)
	Act5C btl crb Dhc64C Disc\RFP Dlic Eb1 egl Gl Lis-1 Mmus\Cd8a Rab35 Scer\GAL4 sn T:Zzzz\eqFP578 wkd γTub37C
Natural transposons (1)
	P-element