Reference Report

Reference
Citation	Ganesan, S., Karr, J.E., Featherstone, D.E. (2011). Drosophila glutamate receptor mRNA expression and mRNP particles. RNA Biol. 8(5): 771--781. (Export to RIS)
FlyBase ID	FBrf0217952
Publication Type	Research paper
PubMed ID	21743295
PubMed Abstract	The processes controlling glutamate receptor expression early in synaptogenesis are poorly understood. Here, we examine glutamate receptor (GluR) subunit mRNA expression and localization in Drosophila embryonic/larval neuromuscular junctions (NMJs). We show that postsynaptic GluR subunit gene expression is triggered by contact from the presynaptic nerve, approximately halfway through embryogenesis. After contact, GluRIIA and GluRIIB mRNA abundance rises quickly approximately 20-fold, then falls within a few hours back to very low levels. Protein abundance, however, gradually increases throughout development. At the same time that mRNA levels decrease following their initial spike, GluRIIA, GluRIIB, and GluRIIC subunit mRNA aggregates become visible in the cytoplasm of postsynaptic muscle cells. These mRNA aggregates do not colocalize with eIF4E, but nevertheless presumably represent mRNP particles of unknown function. Multiplex FISH shows that different GluR subunit mRNAs are found in different mRNPs. GluRIIC mRNPs are most common, followed by GluRIIA and then GluRIIB mRNPs. GluR mRNP density is not increased near NMJs, for any subunit; if anything, GluR mRNP density is highest away from NMJs and near nuclei. These results reveal some of the earliest events in postsynaptic development and provide a foundation for future studies of GluR mRNA biology.
DOI	10.4161/rna.8.5.16014
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	RNA Biol.
Title	RNA Biology.
Publication Year	2004-
ISBN/ISSN	1547-6286 1555-8584
Data from Reference
Aberrations (1)
	Df(2L)Exel8016
Alleles (6)
	Dcr-1^{dsRNA.Scer\UAS.cUa} GluRIIA^AD9 GluRIIA^Scer\UAS.cSa pros¹⁷ Scer\GAL4^how-24B scrt^KG02164
Constructs (2)
	P{UAS-Dcr-1.RNAi.U} P{UAS-GluRIIA.genomic}
Genes (8)
	Dcr-1 eIF-4E GluRIIA GluRIIB GluRIIC pros Scer\GAL4 scrt
Insertions (2)
	P{GawB}how^24B P{SUPor-P}scrt^KG02164
Natural transposons (1)
	P-element