Reference Report

Reference
Citation	Schmidt, R.L., Rinaldo, F.M., Hesse, S.E., Hamada, M., Ortiz, Z., Beleford, D.T., Page-McCaw, A., Platt, J.L., Tang, A.H. (2011). Cleavage of PGRP-LC receptor in the Drosophila IMD pathway in response to live bacterial infection in S2 cells. Self Nonself 2(3): 125--141. (Export to RIS)
FlyBase ID	FBrf0218028
Publication Type	Research paper
PubMed ID	22496930
PubMed Abstract	Drosophila responds to Gram-negative bacterial infection by activating the immune deficiency (IMD) pathway, leading to production of antimicrobial peptides (AMPs). As a receptor for the IMD pathway, peptidoglycan-recognition protein (PGRP), PGRP-LC is known to recognize and bind monomeric peptidoglycan (DAP-type PGN) through its PGRP ectodomain and in turn activate the IMD pathway. The questions remain how PGRP-LC is activated in response to pathogen infection to initiate the IMD signal transduction in Drosophila. Here we present evidence to show that proteases such as elastase and Mmp2 can also activate the IMD pathway but not the TOLL pathway. The elastase-dependent IMD activation requires the receptor PGRP-LC. Importantly, we find that live Salmonella/E. coli infection modulates PGRP-LC expression/receptor integrity and activates the IMD pathway while dead Salmonella/E. coli or protease-deficient E. coli do neither. Our results suggest an interesting possibility that Gram-negative pathogen infection may be partially monitored through the structural integrity of the receptor PGRP-LC via an infection-induced enzyme-based cleavage-mediated activation mechanism.
DOI	10.4161/self.17882
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Self Nonself
Title	Self/nonself
Publication Year
ISBN/ISSN	1938-2030 1938-2049
Data from Reference
Alleles (10)
	Mmp1^{F1.C93A.Scer\UAS} Mmp1^f1.Scer\UAS Mmp1^{F2.C93A.Scer\UAS} Mmp1^F2.Scer\UAS Mmp2^Scer\UAS.cPa PGRP-LC^{I.Scer\UAS.T:Zzzz\FLAG} PGRP-LC^Δ5 PGRP-SA^seml Scer\GAL4^Cg.PA Scer\GAL4^yolk
Constructs (8)
	P{Cg-GAL4.A} P{UAS-Mmp1.F1.C93A} P{UAS-Mmp1.f1} P{UAS-Mmp1.F2.C93A} P{UAS-Mmp1.F2} P{UAS-Mmp2.P} P{UAS-PGRP-LC.I.FLAG} P{yolk-GAL4}
Genes (10)
	AttA CecA1 Def Dpt Drs Mmp1 Mmp2 PGRP-LC PGRP-SA Scer\GAL4
Natural transposons (1)
	P-element