Reference Report

Reference
Citation	Petzoldt, A.G., Coutelis, J.B., Géminard, C., Spéder, P., Suzanne, M., Cerezo, D., Noselli, S. (2012). DE-Cadherin regulates unconventional Myosin ID and Myosin IC in Drosophila left-right asymmetry establishment. Development 139(10): 1874--1884. (Export to RIS)
FlyBase ID	FBrf0218056
Publication Type	Research paper
PubMed ID	22491943
PubMed Abstract	In bilateria, positioning and looping of visceral organs requires proper left-right (L/R) asymmetry establishment. Recent work in Drosophila has identified a novel situs inversus gene encoding the unconventional type ID myosin (MyoID). In myoID mutant flies, the L/R axis is inverted, causing reversed looping of organs, such as the gut, spermiduct and genitalia. We have previously shown that MyoID interacts physically with β-Catenin, suggesting a role of the adherens junction in Drosophila L/R asymmetry. Here, we show that DE-Cadherin co-immunoprecipitates with MyoID and is required for MyoID L/R activity. We further demonstrate that MyoIC, a closely related unconventional type I myosin, can antagonize MyoID L/R activity by preventing its binding to adherens junction components, both in vitro and in vivo. Interestingly, DE-Cadherin inhibits MyoIC, providing a protective mechanism to MyoID function. Conditional genetic experiments indicate that DE-Cadherin, MyoIC and MyoID show temporal synchronicity for their function in L/R asymmetry. These data suggest that following MyoID recruitment by β-Catenin at the adherens junction, DE-Cadherin has a twofold effect on Drosophila L/R asymmetry by promoting MyoID activity and repressing that of MyoIC. Interestingly, the product of the vertebrate situs inversus gene inversin also physically interacts with β-Catenin, suggesting that the adherens junction might serve as a conserved platform for determinants to establish L/R asymmetry both in vertebrates and invertebrates.
DOI	10.1242/dev.047589
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (1)
	Df(3L)920
Alleles (29)
	arm^GD1372 ck^GD1408 d^GD4041 didum^GD1848 jar^GD4044 Mhc^GD1485 Myo10A^GD4042 Myo28B1^GD4043 Myo31DF^{dsRNA.Sym.Scer\UAS} Myo31DF^K2 Myo31DF^Scer\UAS.cSa Myo31DF^{Scer\UAS.T:Avic\GFP} Myo61F^{dsRNA.Scer\UAS} Myo61F^KK101033 Myo61F^Scer\UAS.cHa Myo61F^{Scer\UAS.T:Disc\RFP} Myo95E^GD4040 Myo95E^GD10176 ninaC^GD11545 Scer\GAL4^Abd-B-LDN Scer\GAL4^arm.PS Scer\GAL4^hh.PU Scer\GAL4^{Myo31DF-NP1548} Scer\GAL4^tsh-md621 shg^DN.Scer\UAS shg^KK103334 shg^Scer\UAS.cUa zip^GD1566 α-Cat^GD8808
Constructs (25)
	P{GAL4-arm.S} P{GD1372} P{GD1408} P{GD1485} P{GD1566} P{GD1848} P{GD4040} P{GD4041} P{GD4042} P{GD4043} P{GD4044} P{GD8808} P{GD10176} P{GD11545} P{hh-GAL4.U} P{KK101033} P{KK103334} P{Sym-UAS-Myo31DF} P{UAS-Myo31DF.GFP} P{UAS-Myo31DF.S} P{UAS-Myo61F.dsRNA} P{UAS-Myo61F.H} P{UAS-Myo61F.RFP} P{UAS-shg.DN} P{UAS-shg.U}
Genes (19)
	arm ck d dgo didum en jar Mhc Myo10A Myo28B1 Myo31DF Myo61F Myo95E ninaC Scer\GAL4 shg T:Disc\RFP zip α-Cat
Insertions (3)
	P{GawB}Abd-B^LDN P{GawB}Myo31DF^NP1548 P{GawB}tsh^md621
Natural transposons (1)
	P-element