Reference Report

Reference
Citation	Tunstall, N.E., Herr, A., de Bruyne, M., Warr, C.G. (2012). A Screen for Genes Expressed in the Olfactory Organs of Drosophila melanogaster Identifies Genes Involved in Olfactory Behaviour. PLoS ONE 7(4): e35641. (Export to RIS)
FlyBase ID	FBrf0218110
Publication Type	Research paper
PubMed ID	22530061
PubMed Abstract	For insects the sense of smell and associated olfactory-driven behaviours are essential for survival. Insects detect odorants with families of olfactory receptor proteins that are very different to those of mammals, and there are likely to be other unique genes and genetic pathways involved in the function and development of the insect olfactory system.We have performed a genetic screen of a set of 505 Drosophila melanogaster gene trap insertion lines to identify novel genes expressed in the adult olfactory organs. We identified 16 lines with expression in the olfactory organs, many of which exhibited expression of the trapped genes in olfactory receptor neurons. Phenotypic analysis showed that six of the lines have decreased olfactory responses in a behavioural assay, and for one of these we showed that precise excision of the P element reverts the phenotype to wild type, confirming a role for the trapped gene in olfaction. To confirm the identity of the genes trapped in the lines we performed molecular analysis of some of the insertion sites. While for many lines the reported insertion sites were correct, we also demonstrated that for a number of lines the reported location of the element was incorrect, and in three lines there were in fact two pGT element insertions.We identified 16 new genes expressed in the Drosophila olfactory organs, the majority in neurons, and for several of the gene trap lines demonstrated a defect in olfactory-driven behaviour. Further characterisation of these genes and their roles in olfactory system function and development will increase our understanding of how the insect olfactory system has evolved to perform the same essential function to that of mammals, but using very different molecular genetic mechanisms.
DOI	10.1371/journal.pone.0035641
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Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS ONE
Title	PLoS ONE
Publication Year	2006-
ISBN/ISSN	1932-6203
Data from Reference
Alleles (47)
	A2bp1^BG02836a Aef1^BG01171a CAP^BG02184 CG8771^BG01711 CG42231^BG02820b CG42669^BG02427 CG42699^BG02427 chif^BG02820a CkIIα-i3^BG00973b CkIIα-i3^ex1 CkIIα-i3^ex2 cpo^BG02810 fz2^BG01711 l(2)08717^BG00842 Mctp^BG01140b Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} MYPT-75D^BG01140a Pdk1^BG02759 Pfrx^BG02836b Pka-C1^BG02142 sbb^BG01610 Scer\GAL4^{A2bp1-BG02836a} Scer\GAL4^{Aef1-BG01171a} Scer\GAL4^BG00842 Scer\GAL4^BG02820c Scer\GAL4^CAP-BG02184 Scer\GAL4^{CG8771-BG01711} Scer\GAL4^{CG42231-BG02820b} Scer\GAL4^{CG42669-BG02427} Scer\GAL4^{CG42699-BG02427} Scer\GAL4^{chif-BG02820a} Scer\GAL4^{CkIIα-i3-BG00973b} Scer\GAL4^cpo-BG02810 Scer\GAL4^fz2-BG01711 Scer\GAL4^{Mctp-BG01140b} Scer\GAL4^Pdk1-BG02759 Scer\GAL4^{Pfrx-BG02836b} Scer\GAL4^{Pka-C1-BG02142} Scer\GAL4^sbb-BG01610 Scer\GAL4^shep-BG01322 Scer\GAL4^SKIP-BG00076 Scer\GAL4^tai-BG01746 shep^BG01322 SKIP^BG00076 SKIP^ex1 SKIP^ex2 tai^BG01746
Constructs (2)
	P{GT1} P{UAS-mCD8::GFP.L}
Genes (26)
	A2bp1 Aef1 CAP CG8771 CG12309 CG42231 CG42669 CG42699 chif CkIIα-i3 cpo elav fz2 l(2)08717 Mctp Mmus\Cd8a MYPT-75D Pdk1 Pfrx Pka-C1 sbb Scer\GAL4 shep SKIP tai w
Insertions (25)
	P{GT1}A2bp1^BG02836a P{GT1}Aef1^BG01171a P{GT1}BG00973a P{GT1}BG01171b P{GT1}BG02820c P{GT1}CAP^BG02184 P{GT1}CG8771^BG01711 P{GT1}CG42231^BG02820b P{GT1}CG42669^BG02427 P{GT1}CG42699^BG02427 P{GT1}chif^BG02820a P{GT1}CkIIα-i3^BG00973b P{GT1}cpo^BG02810 P{GT1}fz2^BG01711 P{GT1}l(2)08717^BG00842 P{GT1}Mctp^BG01140b P{GT1}MYPT-75D^BG01140a P{GT1}Pdk1^BG02759 P{GT1}Pfrx^BG02836 P{GT1}Pfrx^BG02836b P{GT1}Pka-C1^BG02142 P{GT1}sbb^BG01610 P{GT1}shep^BG01322 P{GT1}SKIP^BG00076 P{GT1}tai^BG01746