Open Close
Reference
Citation
Magdalena, L.M., Coipan, E.C., Vladimirescu, A.F., Savu, L., Costache, M., Gavrila, L. (2012). Downregulation of hsp22 gene expression in Drosophila melanogaster from sites located near chemical plants.  Genet. Mol. Res. 11(1): 739--745.
FlyBase ID
FBrf0218293
Publication Type
Research paper
Abstract

A common physiological response of organisms to environmental conditions is variation in gene expression, especially true for genes encoding for heat shock proteins. In insects, this process has been examined for induced heat or cold stress. The putative long-term imprinted/acquired heat shock protein response due to unfriendly environmental conditions has been far less studied. The Drosophila melanogaster hsp22 gene, which has been extensively reviewed as being sensitive to different changing life conditions, was examined by qRT-PCR, using carboxy-X-rhodamine. In the present study, we focused on the detection of hsp22 level of transcription in three D. melanogaster isolates, collected from sites located near different chemical plants in Romania and subjected to one-year adaptation to laboratory conditions. In all isolates, the hsp22 gene expression was determined using the housekeeping genes Gapdh1 and UbcD10 as internal controls. According to our experimental results, the D. melanogaster hsp22 gene was significantly downregulated compared to the same gene in w(1118)iso, used as a calibrator. We showed that hsp22 could play an important role in relation to stress resistance and adaptation. This study highlights the importance of in vivo studies to demonstrate genome plasticity to overcome different damages induced by any presumed source of stress.

PubMed ID
PubMed Central ID
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genet. Mol. Res.
    Title
    Genetics and molecular research
    Publication Year
    2002-
    ISBN/ISSN
    1676-5680
    Data From Reference
    Genes (1)