Reference Report

Reference
Citation	Beck, E.S., Gasque, G., Imlach, W.L., Jiao, W., Jiwon Choi, B., Wu, P.S., Kraushar, M.L., McCabe, B.D. (2012). Regulation of Fasciclin II and Synaptic Terminal Development by the Splicing Factor Beag. J. Neurosci. 32(20): 7058--7073. (Export to RIS)
FlyBase ID	FBrf0218385
Publication Type	Research paper
PubMed ID	22593074
PubMed Abstract	Pre-mRNA alternative splicing is an important mechanism for the generation of synaptic protein diversity, but few factors governing this process have been identified. From a screen for Drosophila mutants with aberrant synaptic development, we identified beag, a mutant with fewer synaptic boutons and decreased neurotransmitter release. Beag encodes a spliceosomal protein similar to splicing factors in humans and Caenorhabditis elegans. We find that both beag mutants and mutants of an interacting gene dsmu1 have changes in the synaptic levels of specific splice isoforms of Fasciclin II (FasII), the Drosophila ortholog of neural cell adhesion molecule. We show that restoration of one splice isoform of FasII can rescue synaptic morphology in beag mutants while expression of other isoforms cannot. We further demonstrate that this FasII isoform has unique functions in synaptic development independent of transsynaptic adhesion. beag and dsmu1 mutants demonstrate an essential role for these previously uncharacterized splicing factors in the regulation of synapse development and function.
DOI	10.1523/JNEUROSCI.3717-11.2012
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Neurosci.
Title	Journal of Neuroscience
Publication Year	1981-
ISBN/ISSN	0270-6474 1529-2401
Data from Reference
Aberrations (3)
	Df(1)BSC869 Df(3R)Exel6151 Df(3R)Exel6182
Alleles (29)
	beag¹ beag^EP3260 beag^Scer\UAS.cBa beag^{Scer\UAS.T:Avic\GFP-EYFP} beag^T:Zzzz\FLAG Fas2^C.Scer\UAS Fas2^{dsRNA.Scer\UAS.A} Fas2^e76 Fas2^EB112 Fas2^KK100888 Fas2^{PEST-.RNAiResistant.Scer\UAS} Fas2^{PEST+.AAE.Scer\UAS} Fas2^{PEST+.RNAiResistant.Scer\UAS} Fas2^Scer\UAS.cLa Fas2^Scer\UAS.cLb Fas2^{Scer\UAS.T:Hsap\CD8A} Scer\GAL4^D42 Scer\GAL4^da.G32 Scer\GAL4^elav-C155 Scer\GAL4^G14 Scer\GAL4^OK319 Scer\GAL4^Rapgap1-OK6 Scer\GAL4^unspecified Scer\GAL4^VGlut-OK371 Sh^{Mhc.T:Avic\GFP-S65T,T:Hsap\CD8A} Smu1^f03090 Smu1^Scer\UAS.cBa Smu1^{Scer\UAS.T:Hsap\MYC,T:Disc\RFP} T:Avic\GFP^EYFP
Constructs (17)
	P{beag.Flag} P{GAL4} P{GAL4-da.G32} P{KK100888} P{Mhc.CD8-GFP-Sh} P{UAS.CD8-Fas2} P{UAS-beag.B} P{UAS-beag.EYFP} P{UAS-Fas2.C} P{UAS-Fas2.PEST-.RNAiResistant} P{UAS-Fas2.PEST-} P{UAS-Fas2.PEST+.AAE} P{UAS-Fas2.PEST+.RNAiResistant} P{UAS-Fas2.PEST+} P{UAS-Fas2.RNAi.A} P{UAS-Smu1.B} P{UAS-Smu1.myc.RFP}
Genes (20)
	beag brp Csp Dap160 dlg1 elav Fas2 futsch Mad Nrg Scer\GAL4 Sh Smu1 Syt1 T:Disc\RFP T:Hsap\MYC T:Zzzz\FLAG trol Vap-33-1 wnd
Insertions (8)
	P{EP}beag^EP3260 P{GAL4}G14 P{GawB}D42 P{GawB}elav^C155 P{GawB}OK6 P{GawB}OK319 P{GawB}VGlut^OK371 PBac{WH}Smu1^f03090
Natural transposons (1)
	P-element
Polypeptides (1)
	beag^T:Zzzz\FLAGPA