Reference Report

Reference
Citation	Thistle, R., Cameron, P., Ghorayshi, A., Dennison, L., Scott, K. (2012). Contact Chemoreceptors Mediate Male-Male Repulsion and Male-Female Attraction during Drosophila Courtship. Cell 149(5): 1140--1151. (Export to RIS)
FlyBase ID	FBrf0218411
Publication Type	Research paper
PubMed ID	22632976
PubMed Abstract	The elaborate courtship ritual of Drosophila males is dictated by neural circuitry established by the transcription factor Fruitless and triggered by sex-specific sensory cues. Deciphering the role of different stimuli in driving courtship behavior has been limited by the inability to selectively target appropriate sensory classes. Here, we identify two ion channel genes belonging to the degenerin/epithelial sodium channel/pickpocket (ppk) family, ppk23 and ppk29, which are expressed in fruitless-positive neurons on the legs and are essential for courtship. Gene loss-of-function, cell-inactivation, and cell-activation experiments demonstrate that these genes and neurons are necessary and sufficient to inhibit courtship toward males and promote courtship toward females. Moreover, these cells respond to cuticular hydrocarbons, with different cells selectively responding to male or female pheromones. These studies identify a large population of pheromone-sensing neurons and demonstrate the essential role of contact chemosensation in the early courtship steps of mate selection and courtship initiation.
DOI	10.1016/j.cell.2012.03.045
Related Publication(s)
Supplementary material	Supplemental Information. [FBrf0219192]
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Alleles (23)
	Avic\GFP^{GCaMP3.Scer\UAS.attB} Avic\GFP^{Gr66a.3x.IRES.2x} Ctet\TeTxLC^TNT.Scer\UAS Ecol\lexA^{fru.P1.T:Hsim\VP16} Ecol\lexA^Gr66a fru^P1.LexA Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} ppk23^Scer\UAS.cTa ppk23^Δ8.284 ppk29^Scer\UAS.cTa ppk29^Δ Rnor\CD2^{Ecol\lexAop.T:Avic\GFP} Rnor\CD2^{Scer\UAS.T:Avic\GFP} Scer\GAL4^Gr5a.8.5 Scer\GAL4^Gr32a.3.776 Scer\GAL4^Gr64f.9.7 Scer\GAL4^Gr66a.3.153 Scer\GAL4^Gr68a.PB Scer\GAL4^ppk11.PL Scer\GAL4^ppk23.2.695 Scer\GAL4^ppk28.PC Scer\GAL80^Ecol\lexAop TrpA1^Scer\UAS.cKa
Constructs (18)
	P{Gr5a-GAL4.8.5} P{Gr32a-GAL4.3.776} P{Gr64f-GAL4.9.7} P{Gr66a-GFP.IRES.GFP.IRES.GFP} P{Gr66C1-GAL4.3.153} P{Gr68a-GAL4.B} P{LexAop-Gal80.T} P{lexAop-rCD2-GFP} P{ppk11-GAL4.L} P{ppk23-Gal4.2.695} P{ppk28-GAL4.C} P{UAS-CD2::GFP} P{UAS-GCaMP3.attB} P{UAS-mCD8::GFP.L} P{UAS-ppk23.T} P{UAS-ppk29.T} P{UAS-TeTxLC.tnt} P{UAS-TrpA1.K}
Genes (12)
	Avic\GFP Ctet\TeTxLC Ecol\lexA fru Mmus\Cd8a ppk11 ppk23 ppk29 Rnor\CD2 Scer\GAL4 Scer\GAL80 TrpA1
Transcripts (3)
	Ecol\lexA^{fru.P1.T:Hsim\VP16}RA Scer\GAL4^ppk11.PLRA Scer\GAL4^ppk23.2.695RA