Reference Report

Reference
Citation	Wang, T., Blumhagen, R., Lao, U., Kuo, Y., Edgar, B.A. (2012). LST8 Regulates Cell Growth via Target-of-Rapamycin Complex 2 (TORC2). Mol. Cell. Biol. 32(12): 2203--2213. (Export to RIS)
FlyBase ID	FBrf0218417
Publication Type	Research paper
PubMed ID	22493059
PubMed Abstract	The evolutionarily conserved serine/threonine protein kinase target-of-rapamycin (TOR) controls cell growth as a core component of TOR complexes 1 (TORC1) and 2 (TORC2). Although TORC1 is the more central growth regulator, TORC2 has also been shown to affect cell growth. Here, we demonstrate that Drosophila LST8, the only conserved TOR-binding protein present in both TORC1 and TORC2, functions exclusively in TORC2 and is not required for TORC1 activity. In mutants lacking LST8, expression of TOR and RAPTOR, together with their upstream activator Rheb, was sufficient to provide TORC1 activity and stimulate cell and organ growth. Furthermore, using an lst8 knockout mutation, we show that TORC2 regulates cell growth cell autonomously. Surprisingly, however, TORC2 does not regulate cell growth via its best-characterized target, AKT. Our findings support the possible application of TORC2-specific drugs in cancer therapy.
DOI	10.1128/MCB.06474-11
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Alleles (21)
	Akt1^{T342D.S505D.GMR} Atg8a^{Scer\UAS.T:Avic\GFP} foxo²¹ foxo^Scer\UAS.cPa gig^Scer\UAS.cTa Lst8¹ Lst8³ Lst8^Scer\UAS.cWa Lst8^{Scer\UAS.T:Zzzz\FLAG} raptor^Scer\UAS.cWa raptor^{Scer\UAS.T:Ivir\HA1} Rheb^Scer\UAS.cSa rictor^unspecified Scer\GAL4^da.G32 Scer\GAL4^en.PU Scer\GAL4^GMR.PU Scer\GAL4^tub.PU Scer\GAL80^ts.αTub84B Tor^Scer\UAS.cWa Tor^{Scer\UAS.T:Hsap\MYC} Tsc1^Scer\UAS.cGa
Constructs (17)
	P{en-GAL4.U} P{GAL4-da.G32} P{GMR-Akt1.T342D.S505D} P{GMR-GAL4.U} P{tub-GAL4.U} P{tubP-GAL80^ts} P{UAS-Atg8a.GFP} P{UAS-foxo.P} P{UAS-gig.T} P{UAS-Lst8.flag} P{UAS-Lst8.W} P{UAS-raptor.HA} P{UAS-raptor.W} P{UAS-Rheb.S} P{UAS-Tor.myc} P{UAS-Tor.W} P{UAS-Tsc1.G}
Genes (15)
	Akt1 Atg8a foxo gig Lst8 raptor Rheb rictor S6k Scer\GAL4 Scer\GAL80 T:Hsap\MYC T:Zzzz\FLAG Tor Tsc1
Natural transposons (1)
	P-element