Reference Report

Reference
Citation	Yanagihashi, Y., Usui, T., Izumi, Y., Yonemura, S., Sumida, M., Tsukita, S., Uemura, T., Furuse, M. (2012). Snakeskin, a membrane protein associated with smooth septate junctions, is required for intestinal barrier function in Drosophila. J. Cell Sci. 125(8): 1980--1990. (Export to RIS)
FlyBase ID	FBrf0218431
Publication Type	Research paper
PubMed ID	22328496
PubMed Abstract	Septate junctions (SJs) are the membrane specializations observed between epithelial cells in invertebrates. SJs play a crucial role in epithelial barrier function by restricting the free diffusion of solutes through the intercellular space. In arthropod species, two morphologically different types of SJs have been described: pleated septate junctions (pSJs) and smooth septate junctions (sSJs), which are specific to ectodermal and endodermal epithelia, respectively. In contrast to the recent identification of pSJ-related proteins, the molecular constituents of sSJs are mostly unknown. Here, we report the discovery of a new sSJ-specific membrane protein, designated 'Snakeskin' (Ssk). Ssk is highly concentrated in sSJs in the Drosophila midgut and Malpighian tubules. Lack of Ssk expression is embryonically lethal in Drosophila and results in defective sSJ formation accompanied by abnormal morphology of midgut epithelial cells. We also show that the barrier function of the midgut to a fluorescent tracer is impaired in ssk-knockdown larvae. These results suggest that Ssk is required for the intestinal barrier function in Drosophila.
DOI	10.1242/jcs.096800
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Sci.
Title	Journal of Cell Science
Publication Year	1966-
ISBN/ISSN	0021-9533
Data from Reference
Aberrations (1)
	Df(3L)Ssk
Alleles (5)
	Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^da.G32 Scer\GAL80^ts.αTub84B Ssk^{dsRNA.Scer\UAS} Ssk^Scer\UAS.cYa
Constructs (6)
	P{GAL4-da.G32} P{tubP-GAL80^ts} P{UAS-mCD8::GFP.L} P{UAS-Ssk.RNAi} P{UAS-Ssk.Y} P+PBac{XP5.RB3}
Genes (9)
	CG42674 cora Fas3 kune Mmus\Cd8a Scer\GAL4 Scer\GAL80 Ssk verm
Insertions (3)
	P{XP}d09071 P+PBac{XP5.RB3}Ssk PBac{RB}CG42674^e01995
Natural transposons (1)
	P-element