Koen J. T. Venken, Stacy L. Holtzman, Soo Park, Joseph W. Carlson, Roger A. Hoskins, Hugo J. Bellen, Thomas C. Kaufman The duplications described in this personal communication were generated by first "retrofitting" existing BAC clones using the retrofitting plasmid TKVBL-w+ so that they are competent for Pacman style transgenesis, using the methodology described in Kondo and Perrimon (2009) Cross-species RNAi rescue platform in Drosophila melanogaster. Genetics 183: 1165--1173 (FBrf0209351). The retrofitted clones were then integrated into a docking site on the third chromosome using phiC31-mediated recombination as described in Venken KJ, Popodi E, Holtzman SL, Schulze KL, Park S, Carlson JW, Hoskins RA, Bellen HJ, Kaufman TC. ( 2010) A molecularly defined duplication set for the X chromosome of Drosophila melanogaster. Genetics 186: 1111-1125 (FBrf0212670). The excel spreadsheet listed in the "Associated Files" section of the Reference Report list details for each duplication. Below is some general information that is not provided in the spreadsheet. For each duplication, there are two progenitors (docking site and BAC clone): -The docking site used is listed in the spreadsheet as an abbreviation (VK33), the full name of which is PBac{y+-attP-3B}VK00033 ; FBti0076453. -The second progenitor is the individual BAC clone used to generate each insertion: BACs are listed in the Bac name column and unlike the VK33 site are different for each stock.
