Reference Report

Reference
Citation	Spokony, R., White, K. (2012.5.22). Spokony insertions. (Export to RIS)
FlyBase ID	FBrf0218453
Publication Type	Personal communication to FlyBase
PubMed ID
PubMed Abstract
Text of Personal Communication	The following information and information in a linked spreadsheet accompanied stocks donated to the Bloomington Stock Center by Rebecca Spokony and Kevin White, University of Chicago. BAC clones were recombineered to introduce sequences encoding fluorescence or epitope tags at specific positions within genes to tag specific protein isoforms. The clones were integrated at specific attP sites by phiC31 integrase-mediated transformation. Injections we done in the lab of Thom Kaufman at Indiana University by Stacy Holtzman. Though the constructs are named for the tagged gene, other genes are often present in the constructs. In the construct names, 'GFP.FPTB', 'GFP.FSTF' and 'FTSF.GFP' represent combinations of epitope tags and peptide sequences: GFP.FPTB is a C-terminal superfolderGFP-FLAG-PreScission-TEV-BLRP tag combination. GFP.FSTF is a C-terminal EGFP-FlAsH-StrepII-TEV-3xFlag tag combination. FTSF.GFP is an N-terminal 3xFlag-TEV-StrepII-EGFP-FlAsH tag combination. Information about the protein isoforms tagged by each construct is included in the downloadable spreadsheet. The following insertions are described in the spreadsheet. PBac{Abd-B-EGFP.S}VK00037 PBac{ab-GFP.FLAG}VK00033 PBac{Atf-2-GFP.FLAG}VK00033 PBac{bab2-GFP.FPTB}VK00037 PBac{brk-GFP.FPTB}VK00033 PBac{br-GFP.FPTB}VK00033 PBac{cnc-EGFP.S}VK00037 PBac{CHES-1-like-EGFP.S}VK00033 PBac{dm-GFP.FPTB}VK00033 PBac{disco-r-EGFP.S}VK00033 PBac{dl-GFP.FLAG}VK00033 PBac{Eip74EF-GFP.FLAG}VK00037 PBac{Eip78C-GFP.FLAG}VK00037 PBac{ERR-GFP.FSTF}VK00037 PBac{Ets21C-GFP.FLAG}VK00033 PBac{Ets65A-GFP.FLAG}VK00037 PBac{Ets97D-GFP.FLAG}VK00037 PBac{exd-EGFP.S}VK00033 PBac{eyg-GFP.FPTB}VK00037 PBac{foxo-GFP.FLAG}VK00037 PBac{ftz-f1-GFP.FLAG}VK00037 PBac{gcm2-GFP.FLAG}VK00033 PBac{gcm-GFP.FLAG}VK00033 P{gro-GFP.FLAG}attP16 PBac{Hnf4-GFP.FLAG}VK00033 PBac{Hr51-GFP.FLAG}VK00033 PBac{Hr38-GFP.FLAG}VK00033 PBac{Hr39-GFP.FPTB}VK00033 PBac{Hr78-GFP.FLAG}VK00037 PBac{FTSF.GFP-Hr96}VK00037 PBac{Hr4-GFP.FSTF}VK00033 PBac{jing-GFP.FPTB}VK00033 PBac{kay-GFP.FLAG}VK00037 PBac{kn-GFP.FPTB}VK00033 PBac{lola.BC-GFP.FLAG}VK00033 PBac{lola.J-GFP.FLAG}VK00033 PBac{lola.GR-GFP.FLAG}VK00033 PBac{lola.I-GFP.FLAG}VK00033 PBac{Mad-GFP.FLAG}VK00033 PBac{nmo-GFP.FLAG}VK00037 PBac{N-GFP.FLAG}VK00033 PBac{FTSF.GFP-sens}VK00022 PBac{svp-GFP.FLAG}VK00037 PBac{slou-EGFP.S}VK00037 PBac{Sp1-EGFP.S}VK00033 PBac{Stat92E-GFP.FLAG}VK00037 PBac{tio-GFP.FPTB}VK00033 PBac{usp-GFP.FLAG}VK00033 PBac{vri-GFP.FPTB}VK00033 PBac{zfh2-GFP.FPTB}VK00033
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files	File date: 2012.5.22 ; File size: 26560 ; File format: xlsx ; File name: Spokony.2012.5.22.xlsx
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type
Abbreviation
Title
ISBN/ISSN
Data from Reference
Alleles (52)
	ab^{T:Avic\GFP-SF,T:Zzzz\FLAG} Abd-B^{T:Avic\GFP-EGFP} Atf-2^{T:Avic\GFP-SF,T:Zzzz\FLAG} bab2^{T:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP} br^{T:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP} brk^{T:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP} CHES-1-like^{T:Avic\GFP-EGFP} cnc^{T:Avic\GFP-EGFP} disco-r^{T:Avic\GFP-EGFP} dl^{T:Avic\GFP-SF,T:Zzzz\FLAG} dm^{T:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP} Eip74EF^{T:Avic\GFP-SF,T:Zzzz\FLAG} Eip78C^{T:Avic\GFP-SF,T:Zzzz\FLAG} ERR^{T:Avic\GFP-EGFP,T:Zzzz\TC,T:Zzzz\StrepII,T:Zzzz\TEV.CS,T:Zzzz\FLAG} Ets21C^{T:Avic\GFP-SF,T:Zzzz\FLAG} Ets65A^{T:Avic\GFP-SF,T:Zzzz\FLAG} Ets97D^{T:Avic\GFP-SF,T:Zzzz\FLAG} exd^{T:Avic\GFP-EGFP} eyg^{T:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP} foxo^{T:Avic\GFP-SF,T:Zzzz\FLAG} ftz-f1^{T:Avic\GFP-SF,T:Zzzz\FLAG} gcm2^{T:Avic\GFP-SF,T:Zzzz\FLAG} gcm^{T:Avic\GFP-SF,T:Zzzz\FLAG} gro^{T:Avic\GFP-SF,T:Zzzz\FLAG} Hnf4^{T:Avic\GFP-SF,T:Zzzz\FLAG} Hr4^{T:Avic\GFP-EGFP,T:Zzzz\TC,T:Zzzz\StrepII,T:Zzzz\TEV.CS,T:Zzzz\FLAG} Hr38^{T:Avic\GFP-SF,T:Zzzz\FLAG} Hr39^{C.T:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP} Hr51^{T:Avic\GFP-SF,T:Zzzz\FLAG} Hr78^{T:Avic\GFP-SF,T:Zzzz\FLAG} Hr96^{T:Zzzz\FLAG,T:Zzzz\TEV.CS,T:Zzzz\StrepII,T:Avic\GFP-EGFP,T:Zzzz\TC} jing^{T:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP} kay^{T:Avic\GFP-SF,T:Zzzz\FLAG} kn^{T:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP} lola^{BC.T:Avic\GFP-SF,T:Zzzz\FLAG} lola^{GR.T:Avic\GFP-SF,T:Zzzz\FLAG} lola^{I.T:Avic\GFP-SF,T:Zzzz\FLAG} lola^{J.T:Avic\GFP-SF,T:Zzzz\FLAG} Mad^{T:Avic\GFP-SF,T:Zzzz\FLAG} N^{T:Avic\GFP-SF,T:Zzzz\FLAG} nmo^{T:Avic\GFP-SF,T:Zzzz\FLAG} sens^{T:Zzzz\FLAG,T:Zzzz\TEV.CS,T:Zzzz\StrepII,T:Avic\GFP-EGFP,T:Zzzz\TC} slou^{T:Avic\GFP-EGFP} Sp1^{T:Avic\GFP-EGFP} Stat92E^{T:Avic\GFP-SF,T:Zzzz\FLAG} svp^{T:Avic\GFP-SF,T:Zzzz\FLAG} T:Avic\GFP^EGFP T:Avic\GFP^SF tio^{T:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP} usp^{T:Avic\GFP-SF,T:Zzzz\FLAG} vri^{T:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP} zfh2^{T:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP}
Constructs (50)
	P{gro-GFP.FLAG} PBac{Abd-B-EGFP.S} PBac{ab-GFP.FLAG} PBac{Atf-2-GFP.FLAG} PBac{bab2-GFP.FPTB} PBac{br-GFP.FPTB} PBac{brk-GFP.FPTB} PBac{CHES-1-like-EGFP.S} PBac{cnc-EGFP.S} PBac{disco-r-EGFP.S} PBac{dl-GFP.FLAG} PBac{dm-GFP.FPTB} PBac{Eip74EF-GFP.FLAG} PBac{Eip78C-GFP.FLAG} PBac{ERR-GFP.FSTF} PBac{Ets21C-GFP.FLAG} PBac{Ets65A-GFP.FLAG} PBac{Ets97D-GFP.FLAG} PBac{exd-EGFP.S} PBac{eyg-GFP.FPTB} PBac{foxo-GFP.FLAG} PBac{FTSF.GFP-Hr96} PBac{FTSF.GFP-sens} PBac{ftz-f1-GFP.FLAG} PBac{gcm2-GFP.FLAG} PBac{gcm-GFP.FLAG} PBac{Hnf4-GFP.FLAG} PBac{Hr4-GFP.FSTF} PBac{Hr38-GFP.FLAG} PBac{Hr39-GFP.C.FPTB} PBac{Hr51-GFP.FLAG} PBac{Hr78-GFP.FLAG} PBac{jing-GFP.FPTB} PBac{kay-GFP.FLAG} PBac{kn-GFP.FPTB} PBac{lola.BC-GFP.FLAG} PBac{lola.GR-GFP.FLAG} PBac{lola.I-GFP.FLAG} PBac{lola.J-GFP.FLAG} PBac{Mad-GFP.FLAG} PBac{N-GFP.FLAG} PBac{nmo-GFP.FLAG} PBac{slou-EGFP.S} PBac{Sp1-EGFP.S} PBac{Stat92E-GFP.FLAG} PBac{svp-GFP.FLAG} PBac{tio-GFP.FPTB} PBac{usp-GFP.FLAG} PBac{vri-GFP.FPTB} PBac{zfh2-GFP.FPTB}
Genes (53)
	ab Abd-B Atf-2 bab2 br brk CHES-1-like cnc disco-r dl dm Eip74EF Eip78C ERR Ets21C Ets65A Ets97D exd eyg foxo ftz-f1 gcm gcm2 gro Hnf4 Hr4 Hr38 Hr39 Hr51 Hr78 Hr96 jing kay kn lola Mad N nmo sens slou Sp1 Stat92E svp T:Zzzz\BLRP T:Zzzz\FLAG T:Zzzz\PSP.CS T:Zzzz\StrepII T:Zzzz\TC T:Zzzz\TEV.CS tio usp vri zfh2
Insertions (54)
	P{CaryP}attP16 P{gro-GFP.FLAG}attP16 PBac{Abd-B-EGFP.S}VK00037 PBac{ab-GFP.FLAG}VK00033 PBac{Atf-2-GFP.FLAG}VK00033 PBac{bab2-GFP.FPTB}VK00037 PBac{br-GFP.FPTB}VK00033 PBac{brk-GFP.FPTB}VK00033 PBac{CHES-1-like-EGFP.S}VK00033 PBac{cnc-EGFP.S}VK00037 PBac{disco-r-EGFP.S}VK00033 PBac{dl-GFP.FLAG}VK00033 PBac{dm-GFP.FPTB}VK00033 PBac{Eip74EF-GFP.FLAG}VK00037 PBac{Eip78C-GFP.FLAG}VK00037 PBac{ERR-GFP.FSTF}VK00037 PBac{Ets21C-GFP.FLAG}VK00033 PBac{Ets65A-GFP.FLAG}VK00037 PBac{Ets97D-GFP.FLAG}VK00037 PBac{exd-EGFP.S}VK00033 PBac{eyg-GFP.FPTB}VK00037 PBac{foxo-GFP.FLAG}VK00037 PBac{FTSF.GFP-Hr96}VK00037 PBac{FTSF.GFP-sens}VK00022 PBac{ftz-f1-GFP.FLAG}VK00037 PBac{gcm2-GFP.FLAG}VK00033 PBac{gcm-GFP.FLAG}VK00033 PBac{Hnf4-GFP.FLAG}VK00033 PBac{Hr4-GFP.FSTF}VK00033 PBac{Hr38-GFP.FLAG}VK00033 PBac{Hr39-GFP.C.FPTB}VK00033 PBac{Hr51-GFP.FLAG}VK00033 PBac{Hr78-GFP.FLAG}VK00037 PBac{jing-GFP.FPTB}VK00033 PBac{kay-GFP.FLAG}VK00037 PBac{kn-GFP.FPTB}VK00033 PBac{lola.BC-GFP.FLAG}VK00033 PBac{lola.GR-GFP.FLAG}VK00033 PBac{lola.I-GFP.FLAG}VK00033 PBac{lola.J-GFP.FLAG}VK00033 PBac{Mad-GFP.FLAG}VK00033 PBac{N-GFP.FLAG}VK00033 PBac{nmo-GFP.FLAG}VK00037 PBac{slou-EGFP.S}VK00037 PBac{Sp1-EGFP.S}VK00033 PBac{Stat92E-GFP.FLAG}VK00037 PBac{svp-GFP.FLAG}VK00037 PBac{tio-GFP.FPTB}VK00033 PBac{usp-GFP.FLAG}VK00033 PBac{vri-GFP.FPTB}VK00033 PBac{y⁺-attP-3B}VK00033 PBac{y⁺-attP-3B}VK00037 PBac{y⁺-attP-9A}VK00022 PBac{zfh2-GFP.FPTB}VK00033
Natural transposons (2)
	P-element Tni\piggyBac