Open Close
Reference
Citation
Spokony, R., White, K. (2012.5.22). Spokony insertions. 
FlyBase ID
FBrf0218453
Publication Type
Personal communication to FlyBase
Abstract
PubMed ID
PubMed Central ID
Text of Personal Communication
The following information and information in a linked spreadsheet accompanied stocks donated to the Bloomington Stock Center by Rebecca Spokony and Kevin White, University of Chicago.
BAC clones were recombineered to introduce sequences encoding fluorescence or epitope tags at specific positions within genes to tag specific protein isoforms. The clones were integrated at specific attP sites by phiC31 integrase-mediated transformation. Injections we done in the lab of Thom Kaufman at Indiana University by Stacy Holtzman. Though the constructs are named for the tagged gene, other genes are often present in the constructs. 
In the construct names, 'GFP.FPTB', 'GFP.FSTF' and 'FTSF.GFP' represent combinations of epitope tags and peptide sequences:
GFP.FPTB is a C-terminal superfolderGFP-FLAG-PreScission-TEV-BLRP tag combination. 
GFP.FSTF is a C-terminal EGFP-FlAsH-StrepII-TEV-3xFlag tag combination.
FTSF.GFP is an N-terminal 3xFlag-TEV-StrepII-EGFP-FlAsH tag combination.
Information about the protein isoforms tagged by each construct is included in the downloadable spreadsheet.
The following insertions are described in the spreadsheet.
PBac{Abd-B-EGFP.S}VK00037
PBac{ab-GFP.FLAG}VK00033
PBac{Atf-2-GFP.FLAG}VK00033
PBac{bab2-GFP.FPTB}VK00037
PBac{brk-GFP.FPTB}VK00033
PBac{br-GFP.FPTB}VK00033
PBac{cnc-EGFP.S}VK00037
PBac{CHES-1-like-EGFP.S}VK00033
PBac{dm-GFP.FPTB}VK00033
PBac{disco-r-EGFP.S}VK00033
PBac{dl-GFP.FLAG}VK00033
PBac{Eip74EF-GFP.FLAG}VK00037
PBac{Eip78C-GFP.FLAG}VK00037
PBac{ERR-GFP.FSTF}VK00037
PBac{Ets21C-GFP.FLAG}VK00033
PBac{Ets65A-GFP.FLAG}VK00037
PBac{Ets97D-GFP.FLAG}VK00037
PBac{exd-EGFP.S}VK00033
PBac{eyg-GFP.FPTB}VK00037
PBac{foxo-GFP.FLAG}VK00037
PBac{ftz-f1-GFP.FLAG}VK00037
PBac{gcm2-GFP.FLAG}VK00033
PBac{gcm-GFP.FLAG}VK00033
P{gro-GFP.FLAG}attP16 
PBac{Hnf4-GFP.FLAG}VK00033
PBac{Hr51-GFP.FLAG}VK00033
PBac{Hr38-GFP.FLAG}VK00033
PBac{Hr39-GFP.FPTB}VK00033
PBac{Hr78-GFP.FLAG}VK00037
PBac{FTSF.GFP-Hr96}VK00037
PBac{Hr4-GFP.FSTF}VK00033
PBac{jing-GFP.FPTB}VK00033
PBac{kay-GFP.FLAG}VK00037
PBac{kn-GFP.FPTB}VK00033
PBac{lola.BC-GFP.FLAG}VK00033
PBac{lola.J-GFP.FLAG}VK00033
PBac{lola.GR-GFP.FLAG}VK00033
PBac{lola.I-GFP.FLAG}VK00033
PBac{Mad-GFP.FLAG}VK00033
PBac{nmo-GFP.FLAG}VK00037
PBac{N-GFP.FLAG}VK00033
PBac{FTSF.GFP-sens}VK00022
PBac{svp-GFP.FLAG}VK00037
PBac{slou-EGFP.S}VK00037
PBac{Sp1-EGFP.S}VK00033
PBac{Stat92E-GFP.FLAG}VK00037
PBac{tio-GFP.FPTB}VK00033
PBac{usp-GFP.FLAG}VK00033
PBac{vri-GFP.FPTB}VK00033
PBac{zfh2-GFP.FPTB}VK00033
DOI
Associated Information
Comments
Associated Files
File date: 2012.5.22 ; File size: 26560 ; File format: xlsx ; File name: Spokony.2012.5.22.xlsx
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Abbreviation
    Title
    ISBN/ISSN
    Data From Reference
    Alleles (50)
    Genes (47)
    Natural transposons (2)
    Insertions (54)
    Experimental Tools (8)
    Transgenic Constructs (50)