Reference Report

Reference
Citation	Bolukbasi, E., Vass, S., Cobbe, N., Nelson, B., Simossis, V., Dunbar, D.R., Heck, M.M. (2012). Drosophila poly suggests a novel role for the Elongator complex in insulin receptor-target of rapamycin signalling. Open Biol. 2(1): 110031. (Export to RIS)
FlyBase ID	FBrf0218466
Publication Type	Research paper
PubMed ID	22645656
PubMed Abstract	Multi-cellular organisms need to successfully link cell growth and metabolism to environmental cues during development. Insulin receptor-target of rapamycin (InR-TOR) signalling is a highly conserved pathway that mediates this link. Herein, we describe poly, an essential gene in Drosophila that mediates InR-TOR signalling. Loss of poly results in lethality at the third instar larval stage, but only after a stage of extreme larval longevity. Analysis in Drosophila demonstrates that Poly and InR interact and that poly mutants show an overall decrease in InR-TOR signalling, as evidenced by decreased phosphorylation of Akt, S6K and 4E-BP. Metabolism is altered in poly mutants, as revealed by microarray expression analysis and a decreased triglyceride : protein ratio in mutant animals. Intriguingly, the cellular distribution of Poly is dependent on insulin stimulation in both Drosophila and human cells, moving to the nucleus with insulin treatment, consistent with a role in InR-TOR signalling. Together, these data reveal that Poly is a novel, conserved (from flies to humans) mediator of InR signalling that promotes an increase in cell growth and metabolism. Furthermore, homology to small subunits of Elongator demonstrates a novel, unexpected role for this complex in insulin signalling.
DOI	10.1098/rsob.110031
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Open Biol.
Title	Open biology
Publication Year
ISBN/ISSN	2046-2441
Data from Reference
Alleles (8)
	Akt1⁰⁴²²⁶ InR⁰⁵⁵⁴⁵ poly⁰⁵¹³⁷ poly^Scer\UAS.cBa S6k⁰⁷⁰⁸⁴ Scer\GAL4^Cg.PA Scer\GAL4^GMR.PU Scer\GAL4^hs.PU
Constructs (4)
	P{Cg-GAL4.A} P{GMR-GAL4.U} P{hs-GAL4.U} P{UAS-poly.B}
Genes (17)
	Akt1 CG8036 CG17333 Dic1 His3 InR Lsd-2 Mal-A2 Pgd poly Rm62 S6k Scer\GAL4 Tal Thor TrpA1 Zw
Insertions (1)
	P{PZ}poly⁰⁵¹³⁷
Natural transposons (1)
	P-element