Reference Report

Reference
Citation	Sundram, V., Ng, F.S., Roberts, M.A., Millán, C., Ewer, J., Jackson, F.R. (2012). Cellular Requirements for LARK in the Drosophila Circadian System. J. Biol. Rhythms 27(3): 183--195. (Export to RIS)
FlyBase ID	FBrf0218481
Publication Type	Research paper
PubMed ID	22653887
PubMed Abstract	RNA-binding proteins mediate posttranscriptional functions in the circadian systems of multiple species. A conserved RNA recognition motif (RRM) protein encoded by the lark gene is postulated to serve circadian output and molecular oscillator functions in Drosophila and mammals, respectively. In no species, however, has LARK been eliminated, in vivo, to determine the consequences for circadian timing. The present study utilized RNA interference (RNAi) techniques in Drosophila to decrease LARK levels in clock neurons and other cell types in order to evaluate the circadian functions of the protein. Knockdown of LARK in timeless (TIM)- or pigment dispersing factor (PDF)-containing clock cells caused a significant number of flies to exhibit arrhythmic locomotor activity, demonstrating a requirement for the protein in pacemaker cells. There was no obvious effect on PER protein cycling in lark interference (RNAi) flies, but a knockdown within the PDF neurons was associated with increased PDF immunoreactivity at the dorsal termini of the small ventral lateral neuronal (s-LNv) projections, suggesting an effect on neuropeptide release. The expression of lark RNAi in multiple neurosecretory cell populations demonstrated that LARK is required within pacemaker and nonpacemaker cells for the manifestation of normal locomotor activity rhythms. Interestingly, decreased LARK function in the prothoracic gland (PG), a peripheral organ containing a clock required for the circadian control of eclosion, was associated with weak population eclosion rhythms or arrhythmicity.
DOI	10.1177/0748730412440667
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Biol. Rhythms
Title	Journal of Biological Rhythms
Publication Year	1986-
ISBN/ISSN	0748-7304
Data from Reference
Alleles (18)
	Avic\GFP^Scer\UAS.cUa Dcr-2^Scer\UAS.cDa lark¹ lark^{dsRNA.Scer\UAS.cSa} lark^{dsRNA.Scer\UAS.cSb} lark^NIG.8597R Scer\GAL4^Act5C.PU Scer\GAL4^Ccap.PP Scer\GAL4^dimm-929 Scer\GAL4^Eh.2.4 Scer\GAL4^elav-C155 Scer\GAL4^Mai60 Scer\GAL4^Mai179 Scer\GAL4^nrv2.PS Scer\GAL4^P2.4.Pdf Scer\GAL4^phm.PO Scer\GAL4^tim.Scer\UAS Scer\GAL80^Pdf.PS
Constructs (13)
	P{Act5C-GAL4.U} P{Ccap-GAL4.P} P{GAL4-Eh.2.4} P{NIG.8597R} P{nrv2-GAL4.S} P{Pdf-GAL4.P2.4} P{Pdf-GAL80.S} P{phm-GAL4.O} P{UAS-Dcr-2.D} P{UAS-GFP.U} P{UAS-lark.RNAi.S} P{UAS-lark.RNAi.Sb} P{UAS-tim-GAL4}
Genes (6)
	Avic\GFP Dcr-2 lark Pdf Scer\GAL4 Scer\GAL80
Insertions (4)
	P{GawB}dimm⁹²⁹ P{GawB}elav^C155 P{GawB}l(2)Mai179^Mai179 P{GawB}Mai60
Natural transposons (1)
	P-element