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Citation
Schmidt, I., Thomas, S., Kain, P., Risse, B., Naffin, E., Klämbt, C. (2012). Kinesin heavy chain function in Drosophila glial cells controls neuronal activity.  J. Neurosci. 32(22): 7466--7476.
FlyBase ID
FBrf0218491
Publication Type
Research paper
Abstract
Kinesin heavy chain (Khc) is crucially required for axonal transport and khc mutants show axonal swellings and paralysis. Here, we demonstrate that in Drosophila khc is equally important in glial cells. Glial-specific downregulation of khc by RNA interference suppresses neuronal excitability and results in spastic flies. The specificity of the phenotype was verified by interspecies rescue experiments and further mutant analyses. Khc is mostly required in the subperineurial glia forming the blood-brain barrier. Following glial-specific knockdown, peripheral nerves are swollen with maldistributed mitochondria. To better understand khc function, we determined Khc-dependent Rab proteins in glia and present evidence that Neurexin IV, a well known blood-brain barrier constituent, is one of the relevant cargo proteins. Our work shows that the role of Khc for neuronal excitability must be considered in the light of its necessity for directed transport in glia.
PubMed ID
PubMed Central ID
PMC6703594 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase
Gli-Gal4 line.
Klämbt, 2012.6.22, Gli-Gal4 line. [FBrf0218768]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Neurosci.
    Title
    Journal of Neuroscience
    Publication Year
    1981-
    ISBN/ISSN
    0270-6474 1529-2401
    Data From Reference