Reference Report

Reference
Citation	Tsigkari, K.K., Acevedo, S.F., Skoulakis, E.M. (2012). 14-3-3ε is required for germ cell migration in Drosophila. PLoS ONE 7(5): e36702. (Export to RIS)
FlyBase ID	FBrf0218503
Publication Type	Research paper
PubMed ID	22666326
PubMed Abstract	Although 14-3-3 proteins participate in multiple biological processes, isoform-specific specialized functions, as well as functional redundancy are emerging with tissue and developmental stage-specificity. Accordingly, the two 14-3-3ε proteins in Drosophila exhibit functional specificity and redundancy. Homozygotes for loss of function alleles of D14-3-3ε contain significantly fewer germ line cells (pole cells) in their gonads, a phenotype not shared by mutants in the other 14-3-3 gene leo. We show that although D14-3-3ε is enriched within pole cells it is required in mesodermal somatic gonad precursor cells which guide pole cells in their migration through the mesoderm and coalesce with them to form the embryonic gonad. Loss of D14-3-3ε results in defective pole cell migration, reduced pole cell number. We present evidence that D14-3-3ε loss results in reduction or loss of the transcription factor Zfh-1, one of the main regulatory molecules of the pole cell migration, from the somatic gonad precursor cells.
DOI	10.1371/journal.pone.0036702
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Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS ONE
Title	PLoS ONE
Publication Year	2006-
ISBN/ISSN	1932-6203
Data from Reference
Aberrations (1)
	Df(3R)14-3-3ε^ex24
Alleles (12)
	14-3-3ε^{dsRNA.Scer\UAS.cTa} 14-3-3ε^ex4 14-3-3ε^ex5 14-3-3ε^j2B10 14-3-3ε^Scer\UAS.cCa 14-3-3ζ^I.Scer\UAS 14-3-3ζ^II.Scer\UAS Btau\MAPT^Scer\UAS.cKa Scer\GAL4^Act5C.PI Scer\GAL4^how-24B Scer\GAL4^{nos.UTR.T:Hsim\VP16} Scer\GAL4^αTub84B.PL
Constructs (8)
	P{Act5C-GAL4} P{GAL4::VP16-nos.UTR} P{tubP-GAL4} P{UAS-14-3-3ε.C} P{UAS-14-3-3ε.IR} P{UAS-btau.K} P{UAS-leo.I} P{UAS-leo.II}
Genes (6)
	14-3-3ε 14-3-3ζ Btau\MAPT Scer\GAL4 vas zfh1
Insertions (1)
	P{GawB}how^24B
Natural transposons (2)
	412 P-element