Reference Report

Reference
Citation	Venkei, Z., Przewloka, M.R., Ladak, Y., Albadri, S., Sossick, A., Juhasz, G., Novák, B., Glover, D.M. (2012). Spatiotemporal dynamics of Spc105 regulates the assembly of the Drosophila kinetochore. Open Biol. 2(2): 110032. (Export to RIS)
FlyBase ID	FBrf0218508
Publication Type	Research paper
PubMed ID	22645658
PubMed Abstract	The formation of kinetochores shortly before each cell division is a prerequisite for proper chromosome segregation. The synchronous mitoses of Drosophila syncytial embryos have provided an ideal in vivo system to follow kinetochore assembly kinetics and so address the question of how kinetochore formation is regulated. We found that the nuclear exclusion of the Spc105/KNL1 protein during interphase prevents precocious assembly of the Mis12 complex. The nuclear import of Spc105 in early prophase and its immediate association with the Mis12 complex on centromeres are thus the first steps in kinetochore assembly. The cumulative kinetochore levels of Spc105 and Mis12 complex then determine the rate of Ndc80 complex recruitment commencing only after nuclear envelope breakdown. The carboxy-terminal part of Spc105 directs its nuclear import and is sufficient for the assembly of all core kinetochore components and CENP-C, when localized ectopically to centrosomes. Super-resolution microscopy shows that carboxy-terminus of Spc105 lies at the junction of the Mis12 and Ndc80 complexes on stretched kinetochores. Our study thus indicates that physical accessibility of kinetochore components plays a crucial role in the regulation of Drosophila kinetochore assembly and leads us to a model in which Spc105 is a licensing factor for its onset.
DOI	10.1098/rsob.110032
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Open Biol.
Title	Open biology
Publication Year
ISBN/ISSN	2046-2441
Data from Reference
Alleles (7)
	Kmn1^{Scer\UAS.P\T.C.T:Avic\GFP-EGFP} Mis12^{Scer\UAS.P\T.C.T:Avic\GFP-EGFP} Nuf2^{Scer\UAS.P\T.C.T:Avic\GFP-EGFP} Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} Spc25^{Scer\UAS.cVa.T:Avic\GFP-EGFP} Spc105R^{Scer\UAS.P\T.C.T:Avic\GFP-EGFP} T:Avic\GFP^EGFP
Constructs (6)
	P{matα4-GAL-VP16} P{UASp-Kmn1-EGFP} P{UASp-Mis12-EGFP} P{UASp-Nuf2-EGFP} P{UASp-Spc105R-GFP} P{UAS-Spc25.EGFP.V}
Genes (11)
	Cenp-C cid cp309 His3 Kmn1 Mis12 Ndc80 Nuf2 Scer\GAL4 Spc25 Spc105R
Natural transposons (1)
	P-element